Impaired gamma interferon production by cells from patients with lymphoproliferative disorders of mature T and NK cells

F. Pandolfi, M. Capobianchi, P. Matricardi, J. Facchini, G. Bonomo, G. De Rossi, G. Semenzato, M. Fiorilli, F. Dianzani, F. Aiuti

Research output: Contribution to journalArticlepeer-review

Abstract

We investigated interferon (IFN) production by peripheral blood mononuclear cells from four patients with chronic OKT4 T-lymphocytic leukaemia and three patients with abnormal expansions of granular lymphocytes. No spontaneous production of IFN-γ was found in wupernatants of cultures from both patients and normal controls. However, whereas the enzyme galactose oxidase or staphylococcal enterotoxin B was able to induce IFN-γ production by normal cells, no production could be obtained in the cells under study. The possibility that this lack of production might have been attributed to an excess of N-acetylneuramic acid masking galactose residues or to a defect of monocyte accessory cells was ruled out either by pre-treating the cells with neuraminidase or by adding normal adherent cells to the cultures, both of which resulted in a lack of production. On the contrary, the calcium ionophore A23187 (considered to act as a second specific step, following oxidation of galactose residues, toward genetic derepression of IFN-γ) induced considerable IFN-γ production in all the three tested patients. It can be concluded that, although in rare cases, as previously reported by other authors, cells from patients with T or NK lymphoproliferative disorders may spontaneously produce IFN-γ, this is not a general mechanism that underlies the disease. In fact, in all our cases a defect of IFN-γ production was found. This defect seems due to an alteration at the membrane level of the galactose-containing glycoproteins and can be restored by induced with a calcium ionophore.

Original languageEnglish
Pages (from-to)315-320
Number of pages6
JournalScandinavian Journal of Immunology
Volume21
Issue number4
DOIs
Publication statusPublished - 1985

ASJC Scopus subject areas

  • Immunology

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