Impaired hepatic mitochondrial oxidation using the 13C-methionine breath test in patients with macrovesicular steatosis and patients with cirrhosis

Laurent Spahr, Francesco Negro, Gioacchino Leandro, Oana Marinescu, Keith J. Goodman, Laura Rubbia-Brandt, Martha Jordan, Antoine Hadengue

Research output: Contribution to journalArticlepeer-review

Abstract

Background: In cirrhosis, hepatic mitochondriae exhibit morphological and functional abnormalities. In human steatosis, ultrastructural changes are reported in the absence of ethanol. Routine evaluation of mitochondrial function is difficult. We used a breath test to explore hepatic mitochondrial oxidation in vivo. Material/Methods: The 13C-methionine breath test was performed in healthy subjects (n=15), patients with cirrhosis (n=25), and patients with biopsy-proven severe (> 40% involved hepatocytes), non-alcoholic macrovesicular steatosis (n=18). After oral administration of 13C-methionine (200 mg), hepatic mitochondrial decarboxylation was measured by breath [13C]-CO2 enrichment, expressed as dose/h and delta over base (DOB), for 1 hour, by isotope ratio infrared spectroscopy. Results were normalized against BM1. Results: At 60 minutes, patients with steatosis had reduced exhalation of 13CO2 as compared to healthy subjects (dose/h: -47%, 18.8±12 vs 36±6.1; DOB: -52%, 40.4±32 vs 85±20, p13C-methionine breath test is impaired both in patients with pure non alcohol-related severe macrovesicular steatosis and in patients with cirrhosis of mixed etiologies. This non-invasive test can be used to monitor hepatic mitochondrial function in vivo.

Original languageEnglish
JournalMedical Science Monitor
Volume9
Issue number1
Publication statusPublished - Jan 1 2003

Keywords

  • Cirrhosis
  • Liver biopsy
  • Methionine
  • Obesity

ASJC Scopus subject areas

  • Medicine(all)

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