Impaired in vitro growth of peripheral blood hematopoietic progenitor cells in HIV-infected patients: Evidence of an inhibitory effect of autologous T lymphocytes

E. Balleari, S. Timitilli, F. Puppo, L. Gaffuri, C. Musselli, F. Rizzo, F. Indiveri, R. Ghio

Research output: Contribution to journalArticle

Abstract

The in vitro growth of both circulating granulocyte-macrophage (CFU-GM) and erythroid (BFU-E) progenitor cells was assessed in 29 individuals infected with the human immunodeficiency virus (HIV) at different stages of infection. The effects of autologous T lymphocytes, adherent cells, and serum on the in vitro growth of hematopoietic progenitor cells were also investigated. Compared with normal controls, baseline growth was significantly reduced for both CFU-GM and BFU-E in HIV-infected patients, independent of the clinical stage of the disease. In HIV-infected subjects depletion of autologous T cells was associated with a significant increase of progenitor cell growth. Similar results were observed after selective depletion of CD 8 + T cells, while readdition of T cells to T-depleted mononuclear cells reduced the number of CFU-GM. A dose-dependent colony inhibitory activity was found in conditioned medium of T cells from HIV-infected subjects. Neither autologous adherent cells nor serum from either HIV+ or HIV§ubjects had any significant effect on the in vitro growth of CFU-GM. These results indicate that the in vitro growth of circulating hematopoietic progenitor cells is impaired even in the early stages of HIV infection, and that autologous T cells exert an inhibitory effect on the in vitro growth of progenitor cells, possibly via soluble mediator(s).

Original languageEnglish
Pages (from-to)320-325
Number of pages6
JournalAnnals of Hematology
Volume63
Issue number6
DOIs
Publication statusPublished - Dec 1991

Keywords

  • AIDS
  • hemato
  • Human immunodeficiency virus
  • poietic progenitor cells
  • T-lymphocyte subsets

ASJC Scopus subject areas

  • Hematology

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