Impaired in vitro growth of peripheral blood hematopoietic progenitor cells in HIV-infected patients: Evidence of an inhibitory effect of autologous T lymphocytes

E. Balleari, S. Timitilli, F. Puppo, L. Gaffuri, C. Musselli, F. Rizzo, F. Indiveri, R. Ghio

Research output: Contribution to journalArticle

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Abstract

The in vitro growth of both circulating granulocyte-macrophage (CFU-GM) and erythroid (BFU-E) progenitor cells was assessed in 29 individuals infected with the human immunodeficiency virus (HIV) at different stages of infection. The effects of autologous T lymphocytes, adherent cells, and serum on the in vitro growth of hematopoietic progenitor cells were also investigated. Compared with normal controls, baseline growth was significantly reduced for both CFU-GM and BFU-E in HIV-infected patients, independent of the clinical stage of the disease. In HIV-infected subjects depletion of autologous T cells was associated with a significant increase of progenitor cell growth. Similar results were observed after selective depletion of CD 8 + T cells, while readdition of T cells to T-depleted mononuclear cells reduced the number of CFU-GM. A dose-dependent colony inhibitory activity was found in conditioned medium of T cells from HIV-infected subjects. Neither autologous adherent cells nor serum from either HIV+ or HIV§ubjects had any significant effect on the in vitro growth of CFU-GM. These results indicate that the in vitro growth of circulating hematopoietic progenitor cells is impaired even in the early stages of HIV infection, and that autologous T cells exert an inhibitory effect on the in vitro growth of progenitor cells, possibly via soluble mediator(s).

Original languageEnglish
Pages (from-to)320-325
Number of pages6
JournalAnnals of Hematology
Volume63
Issue number6
DOIs
Publication statusPublished - Dec 1991

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Hematopoietic Stem Cells
HIV
Granulocyte-Macrophage Progenitor Cells
T-Lymphocytes
Growth
Erythroid Precursor Cells
Stem Cells
Virus Diseases
Conditioned Culture Medium
In Vitro Techniques
Serum
Granulocytes
Cell Count
Macrophages
Infection

Keywords

  • AIDS
  • hemato
  • Human immunodeficiency virus
  • poietic progenitor cells
  • T-lymphocyte subsets

ASJC Scopus subject areas

  • Hematology

Cite this

Impaired in vitro growth of peripheral blood hematopoietic progenitor cells in HIV-infected patients : Evidence of an inhibitory effect of autologous T lymphocytes. / Balleari, E.; Timitilli, S.; Puppo, F.; Gaffuri, L.; Musselli, C.; Rizzo, F.; Indiveri, F.; Ghio, R.

In: Annals of Hematology, Vol. 63, No. 6, 12.1991, p. 320-325.

Research output: Contribution to journalArticle

Balleari, E. ; Timitilli, S. ; Puppo, F. ; Gaffuri, L. ; Musselli, C. ; Rizzo, F. ; Indiveri, F. ; Ghio, R. / Impaired in vitro growth of peripheral blood hematopoietic progenitor cells in HIV-infected patients : Evidence of an inhibitory effect of autologous T lymphocytes. In: Annals of Hematology. 1991 ; Vol. 63, No. 6. pp. 320-325.
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