Impaired nerve-mediated relaxation of penile tissue from caveolin-1 deficient mice

Yulia Shakirova, Petter Hedlund, Karl Swärd

Research output: Contribution to journalArticle

Abstract

Caveolin-1-deficient mice are characterised by a high vascular NO production. Because NO-dependent smooth muscle relaxation is considered to play an important role in penile erection, it was hypothesized that the erectile function would be affected by genetic ablation of caveolae. This study assessed penile erectile mechanisms in caveolin-1 knockout (KO) mice ex vivo. Immunofluorescence confirmed caveolin-1 expression primarily in the endothelium surrounding the sinusoids of the corpus cavernosum, but also in smooth muscle cells of the sinusoidal bundles. In KO mice, caveolin-1 was absent, and the expression of the caveola-associated protein PTRF-Cavin was reduced. Nitric oxide synthase (endothelial and neuronal) and caveolin-3 levels were not affected, and staining of the neuronal marker PGP 9.5 did not disclose any apparent change in the density or pattern of innervation. Moreover, no apparent morphological differences were noted. Functionally, the force response following stimulation of α 1-adrenergic receptors, and the sensitivity to the Rho-kinase inhibitor Y27632, were unaltered, whereas relaxation of α 1-precontracted corpus cavernosum in response to electrical field stimulation and the muscarinic agonist carbachol were impaired. The nitric oxide donor sodium nitroprusside produced less relaxation in KO as compared to wild type corpus cavernosum. We conclude that nerve-mediated dilatation of the corpus cavernosum is impaired in the absence of caveolin-1, and that this is due in part to reduced sensitivity of the target tissue to NO. All in all our data support an important role of caveolin-1 in penile erection.

Original languageEnglish
Pages (from-to)399-405
Number of pages7
JournalEuropean Journal of Pharmacology
Volume602
Issue number2-3
DOIs
Publication statusPublished - Jan 14 2009

Fingerprint

Caveolin 1
Penile Erection
Caveolae
Knockout Mice
Caveolin 3
Muscarinic Agonists
rho-Associated Kinases
Nitric Oxide Synthase Type I
Muscle Relaxation
Nitric Oxide Donors
Nitric Oxide Synthase Type III
Carbachol
Nitroprusside
Adrenergic Receptors
Electric Stimulation
Smooth Muscle Myocytes
Endothelium
Fluorescent Antibody Technique
Smooth Muscle
Blood Vessels

Keywords

  • Caveolae
  • Guanylate cyclase
  • Nitric oxide
  • Penile erection
  • Phosphodiesterase
  • Physiology

ASJC Scopus subject areas

  • Pharmacology

Cite this

Impaired nerve-mediated relaxation of penile tissue from caveolin-1 deficient mice. / Shakirova, Yulia; Hedlund, Petter; Swärd, Karl.

In: European Journal of Pharmacology, Vol. 602, No. 2-3, 14.01.2009, p. 399-405.

Research output: Contribution to journalArticle

Shakirova, Yulia ; Hedlund, Petter ; Swärd, Karl. / Impaired nerve-mediated relaxation of penile tissue from caveolin-1 deficient mice. In: European Journal of Pharmacology. 2009 ; Vol. 602, No. 2-3. pp. 399-405.
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