Impaired surfactant protein B synthesis in infants with congenital diaphragmatic hernia

Paola E. Cogo; Manuela Simonato; Olivier Danhaive; Giovanna Verlato; Giovanna Cobellis; Francesco Savignoni; Donatella Peca; Aldo Baritussio; Virgilio P. Carnielli

doi:10.1183/09031936.00032212

Impaired surfactant protein B synthesis in infants with congenital diaphragmatic hernia

Paola E. Cogo, Manuela Simonato, Olivier Danhaive, Giovanna Verlato, Giovanna Cobellis, Francesco Savignoni, Donatella Peca, Aldo Baritussio, Virgilio P. Carnielli

Ospedale Pediatrico Bambino Gesu

Research output: Contribution to journal › Article › peer-review

Abstract

Pulmonary hypoplasia and hypertension account for significant morbidity and mortality in neonates with congenital diaphragmatic hernia (CDH). Whether CDH is associated with surfactant dysfunction remains controversial. Therefore, we measured disaturated phosphatidylcholine (DSPC) and surfactant protein (SP)-B concentration in tracheal aspirates and their synthesis rate in infants with CDH compared to infants without lung disease. 2H2O as a precursor of DSPC and 1-13C-leucine as a precursor of SP-B were administered to 13 infants with CDH and eight controls matched for gestational age. DSPC and SP-B were isolated from tracheal aspirates, and their fractional synthesis rate was derived from 2H and 13C enrichment curves obtained by mass spectrometry. DSPC and SP-B amounts in tracheal aspirates were also measured. In infants with CDH, SP-B fractional synthesis rate and amount were 62±27% and 57±22% lower, respectively, than the value found in infants without lung disease (p

Original language	English
Pages (from-to)	677-682
Number of pages	6
Journal	European Respiratory Journal
Volume	41
Issue number	3
DOIs	https://doi.org/10.1183/09031936.00032212
Publication status	Published - Mar 1 2013

Keywords

Isotope labelling
Metabolism
Pulmonary surfactants

ASJC Scopus subject areas

Pulmonary and Respiratory Medicine

Access to Document

10.1183/09031936.00032212

Fingerprint Dive into the research topics of 'Impaired surfactant protein B synthesis in infants with congenital diaphragmatic hernia'. Together they form a unique fingerprint.

Cite this

Impaired surfactant protein B synthesis in infants with congenital diaphragmatic hernia. / Cogo, Paola E.; Simonato, Manuela; Danhaive, Olivier; Verlato, Giovanna; Cobellis, Giovanna; Savignoni, Francesco; Peca, Donatella; Baritussio, Aldo; Carnielli, Virgilio P.

In: European Respiratory Journal, Vol. 41, No. 3, 01.03.2013, p. 677-682.

Research output: Contribution to journal › Article › peer-review

@article{97d1e04775c744969de389b4171a6bae,

title = "Impaired surfactant protein B synthesis in infants with congenital diaphragmatic hernia",

abstract = "Pulmonary hypoplasia and hypertension account for significant morbidity and mortality in neonates with congenital diaphragmatic hernia (CDH). Whether CDH is associated with surfactant dysfunction remains controversial. Therefore, we measured disaturated phosphatidylcholine (DSPC) and surfactant protein (SP)-B concentration in tracheal aspirates and their synthesis rate in infants with CDH compared to infants without lung disease. 2H2O as a precursor of DSPC and 1-13C-leucine as a precursor of SP-B were administered to 13 infants with CDH and eight controls matched for gestational age. DSPC and SP-B were isolated from tracheal aspirates, and their fractional synthesis rate was derived from 2H and 13C enrichment curves obtained by mass spectrometry. DSPC and SP-B amounts in tracheal aspirates were also measured. In infants with CDH, SP-B fractional synthesis rate and amount were 62±27% and 57±22% lower, respectively, than the value found in infants without lung disease (p",

keywords = "Isotope labelling, Metabolism, Pulmonary surfactants",

author = "Cogo, {Paola E.} and Manuela Simonato and Olivier Danhaive and Giovanna Verlato and Giovanna Cobellis and Francesco Savignoni and Donatella Peca and Aldo Baritussio and Carnielli, {Virgilio P.}",

year = "2013",

month = mar,

day = "1",

doi = "10.1183/09031936.00032212",

language = "English",

volume = "41",

pages = "677--682",

journal = "European Journal of Respiratory Diseases",

issn = "0903-1936",

publisher = "European Respiratory Society",

number = "3",

}

TY - JOUR

T1 - Impaired surfactant protein B synthesis in infants with congenital diaphragmatic hernia

AU - Cogo, Paola E.

AU - Simonato, Manuela

AU - Danhaive, Olivier

AU - Verlato, Giovanna

AU - Cobellis, Giovanna

AU - Savignoni, Francesco

AU - Peca, Donatella

AU - Baritussio, Aldo

AU - Carnielli, Virgilio P.

PY - 2013/3/1

Y1 - 2013/3/1

N2 - Pulmonary hypoplasia and hypertension account for significant morbidity and mortality in neonates with congenital diaphragmatic hernia (CDH). Whether CDH is associated with surfactant dysfunction remains controversial. Therefore, we measured disaturated phosphatidylcholine (DSPC) and surfactant protein (SP)-B concentration in tracheal aspirates and their synthesis rate in infants with CDH compared to infants without lung disease. 2H2O as a precursor of DSPC and 1-13C-leucine as a precursor of SP-B were administered to 13 infants with CDH and eight controls matched for gestational age. DSPC and SP-B were isolated from tracheal aspirates, and their fractional synthesis rate was derived from 2H and 13C enrichment curves obtained by mass spectrometry. DSPC and SP-B amounts in tracheal aspirates were also measured. In infants with CDH, SP-B fractional synthesis rate and amount were 62±27% and 57±22% lower, respectively, than the value found in infants without lung disease (p

AB - Pulmonary hypoplasia and hypertension account for significant morbidity and mortality in neonates with congenital diaphragmatic hernia (CDH). Whether CDH is associated with surfactant dysfunction remains controversial. Therefore, we measured disaturated phosphatidylcholine (DSPC) and surfactant protein (SP)-B concentration in tracheal aspirates and their synthesis rate in infants with CDH compared to infants without lung disease. 2H2O as a precursor of DSPC and 1-13C-leucine as a precursor of SP-B were administered to 13 infants with CDH and eight controls matched for gestational age. DSPC and SP-B were isolated from tracheal aspirates, and their fractional synthesis rate was derived from 2H and 13C enrichment curves obtained by mass spectrometry. DSPC and SP-B amounts in tracheal aspirates were also measured. In infants with CDH, SP-B fractional synthesis rate and amount were 62±27% and 57±22% lower, respectively, than the value found in infants without lung disease (p

KW - Isotope labelling

KW - Metabolism

KW - Pulmonary surfactants

UR - http://www.scopus.com/inward/record.url?scp=84875275057&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84875275057&partnerID=8YFLogxK

U2 - 10.1183/09031936.00032212

DO - 10.1183/09031936.00032212

M3 - Article

C2 - 22700843

AN - SCOPUS:84875275057

VL - 41

SP - 677

EP - 682

JO - European Journal of Respiratory Diseases

JF - European Journal of Respiratory Diseases

SN - 0903-1936

IS - 3

ER -