Impaired vasorelaxant responses to natriuretic peptides in the stroke-prone phenotype of spontaneously hypertensive rats

Rosaria Russo, Carmine Vecchione, Francesco Cosentino, Armando Natale, Alessandro F. Mele, Miriam Muscolo, Carmine Savoia, Detlev Ganten, Speranza Rubattu, Massimo Volpe

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12 Citations (Scopus)

Abstract

Background: We have previously shown that a locus on rat chromosome 5, termed STR 2, co-localizes with the genes encoding atrial natriuretic and brain natriuretic peptides, and is closely linked to the development of strokes in rats of a F 2 hybrid cohort obtained by crossing stroke-prone spontaneously hypertensive rats and spontaneously hypertensive rats. We also demonstrated that there are significant differences in vascular functioning that are co-segregated with stroke latency of stroke-prone spontaneously hypertensive rats. Objective: To investigate the vascular responses to natriuretic peptides in the stroke-prone phenotype of spontaneously hypertensive rats. Design and methods: In view of the important vasoactive properties of natriuretic peptides, we tested the vascular responses to 10 -11-10 -9 mol/l atrial natriuretic peptide and to 10 -11-10 -7 mol/l brain natriuretic peptide in isolated rings of aortas and internal carotid arteries obtained from stroke-prone and stroke-resistant spontaneously hypertensive rats. The 6-week-old rats were exposed for 4 weeks either to their regular diet (n = 15 of both strains) or to the stroke-permissive Japanese-style diet (n = 14 of both strains). A group of 14 normotensive, age-matched and sex-matched Wistar-Kyoto rats was also studied. Results: Systolic blood pressures in stroke-prone and stroke-resistant spontaneously hypertensive rats were similar, and were significantly higher than those in Wistar-Kyoto rats. Vascular responses to nitroglycerin, atrial natriuretic peptide, and brain natriuretic peptide in rats of the two hypertensive strains and in Wistar-Kyoto rats fed their regular diet were comparable. In contrast, the vasorelaxant responses to atrial natriuretic peptide in stroke-prone spontaneously hypertensive rats fed Japanese diet were lower both in aortas and in internal carotid arteries than were those in spontaneously hypertensive rats (both P <0.05 by analysis of variance) and in Wistar-Kyoto rats (both P <0.05). Similarly, vasorelaxant responses to brain natriuretic peptide were lower both in aortas and in internal carotid arteries of stroke-prone spontaneously hypertensive rats than they were in spontaneously hypertensive rats (both P <0.05) and in Wistar-Kyoto rats (P <0.05). The responses to nitroglycerin in the stroke-prone spontaneously hypertensive rats and spontaneously hypertensive rats fed Japanese-style diet were also similar. Conclusion: The vasorelaxant effects of natriuretic peptides are impaired in stroke-prone spontaneously hypertensive rats. This abnormality could play a role in the pathogenesis of stroke incidence in this hypertensive model.

Original languageEnglish
Pages (from-to)151-156
Number of pages6
JournalJournal of Hypertension
Volume16
Issue number2
DOIs
Publication statusPublished - 1998

Fingerprint

Natriuretic Peptides
Inbred SHR Rats
Vasodilator Agents
Stroke
Phenotype
Inbred WKY Rats
Blood Vessels
Brain Natriuretic Peptide
Diet
Internal Carotid Artery
Atrial Natriuretic Factor
Aorta
Nitroglycerin
Blood Pressure
Chromosomes, Human, Pair 5

Keywords

  • Endothelium
  • Natriuretic peptides
  • Strokes

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology

Cite this

Impaired vasorelaxant responses to natriuretic peptides in the stroke-prone phenotype of spontaneously hypertensive rats. / Russo, Rosaria; Vecchione, Carmine; Cosentino, Francesco; Natale, Armando; Mele, Alessandro F.; Muscolo, Miriam; Savoia, Carmine; Ganten, Detlev; Rubattu, Speranza; Volpe, Massimo.

In: Journal of Hypertension, Vol. 16, No. 2, 1998, p. 151-156.

Research output: Contribution to journalArticle

Russo, Rosaria ; Vecchione, Carmine ; Cosentino, Francesco ; Natale, Armando ; Mele, Alessandro F. ; Muscolo, Miriam ; Savoia, Carmine ; Ganten, Detlev ; Rubattu, Speranza ; Volpe, Massimo. / Impaired vasorelaxant responses to natriuretic peptides in the stroke-prone phenotype of spontaneously hypertensive rats. In: Journal of Hypertension. 1998 ; Vol. 16, No. 2. pp. 151-156.
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title = "Impaired vasorelaxant responses to natriuretic peptides in the stroke-prone phenotype of spontaneously hypertensive rats",
abstract = "Background: We have previously shown that a locus on rat chromosome 5, termed STR 2, co-localizes with the genes encoding atrial natriuretic and brain natriuretic peptides, and is closely linked to the development of strokes in rats of a F 2 hybrid cohort obtained by crossing stroke-prone spontaneously hypertensive rats and spontaneously hypertensive rats. We also demonstrated that there are significant differences in vascular functioning that are co-segregated with stroke latency of stroke-prone spontaneously hypertensive rats. Objective: To investigate the vascular responses to natriuretic peptides in the stroke-prone phenotype of spontaneously hypertensive rats. Design and methods: In view of the important vasoactive properties of natriuretic peptides, we tested the vascular responses to 10 -11-10 -9 mol/l atrial natriuretic peptide and to 10 -11-10 -7 mol/l brain natriuretic peptide in isolated rings of aortas and internal carotid arteries obtained from stroke-prone and stroke-resistant spontaneously hypertensive rats. The 6-week-old rats were exposed for 4 weeks either to their regular diet (n = 15 of both strains) or to the stroke-permissive Japanese-style diet (n = 14 of both strains). A group of 14 normotensive, age-matched and sex-matched Wistar-Kyoto rats was also studied. Results: Systolic blood pressures in stroke-prone and stroke-resistant spontaneously hypertensive rats were similar, and were significantly higher than those in Wistar-Kyoto rats. Vascular responses to nitroglycerin, atrial natriuretic peptide, and brain natriuretic peptide in rats of the two hypertensive strains and in Wistar-Kyoto rats fed their regular diet were comparable. In contrast, the vasorelaxant responses to atrial natriuretic peptide in stroke-prone spontaneously hypertensive rats fed Japanese diet were lower both in aortas and in internal carotid arteries than were those in spontaneously hypertensive rats (both P <0.05 by analysis of variance) and in Wistar-Kyoto rats (both P <0.05). Similarly, vasorelaxant responses to brain natriuretic peptide were lower both in aortas and in internal carotid arteries of stroke-prone spontaneously hypertensive rats than they were in spontaneously hypertensive rats (both P <0.05) and in Wistar-Kyoto rats (P <0.05). The responses to nitroglycerin in the stroke-prone spontaneously hypertensive rats and spontaneously hypertensive rats fed Japanese-style diet were also similar. Conclusion: The vasorelaxant effects of natriuretic peptides are impaired in stroke-prone spontaneously hypertensive rats. This abnormality could play a role in the pathogenesis of stroke incidence in this hypertensive model.",
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author = "Rosaria Russo and Carmine Vecchione and Francesco Cosentino and Armando Natale and Mele, {Alessandro F.} and Miriam Muscolo and Carmine Savoia and Detlev Ganten and Speranza Rubattu and Massimo Volpe",
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T1 - Impaired vasorelaxant responses to natriuretic peptides in the stroke-prone phenotype of spontaneously hypertensive rats

AU - Russo, Rosaria

AU - Vecchione, Carmine

AU - Cosentino, Francesco

AU - Natale, Armando

AU - Mele, Alessandro F.

AU - Muscolo, Miriam

AU - Savoia, Carmine

AU - Ganten, Detlev

AU - Rubattu, Speranza

AU - Volpe, Massimo

PY - 1998

Y1 - 1998

N2 - Background: We have previously shown that a locus on rat chromosome 5, termed STR 2, co-localizes with the genes encoding atrial natriuretic and brain natriuretic peptides, and is closely linked to the development of strokes in rats of a F 2 hybrid cohort obtained by crossing stroke-prone spontaneously hypertensive rats and spontaneously hypertensive rats. We also demonstrated that there are significant differences in vascular functioning that are co-segregated with stroke latency of stroke-prone spontaneously hypertensive rats. Objective: To investigate the vascular responses to natriuretic peptides in the stroke-prone phenotype of spontaneously hypertensive rats. Design and methods: In view of the important vasoactive properties of natriuretic peptides, we tested the vascular responses to 10 -11-10 -9 mol/l atrial natriuretic peptide and to 10 -11-10 -7 mol/l brain natriuretic peptide in isolated rings of aortas and internal carotid arteries obtained from stroke-prone and stroke-resistant spontaneously hypertensive rats. The 6-week-old rats were exposed for 4 weeks either to their regular diet (n = 15 of both strains) or to the stroke-permissive Japanese-style diet (n = 14 of both strains). A group of 14 normotensive, age-matched and sex-matched Wistar-Kyoto rats was also studied. Results: Systolic blood pressures in stroke-prone and stroke-resistant spontaneously hypertensive rats were similar, and were significantly higher than those in Wistar-Kyoto rats. Vascular responses to nitroglycerin, atrial natriuretic peptide, and brain natriuretic peptide in rats of the two hypertensive strains and in Wistar-Kyoto rats fed their regular diet were comparable. In contrast, the vasorelaxant responses to atrial natriuretic peptide in stroke-prone spontaneously hypertensive rats fed Japanese diet were lower both in aortas and in internal carotid arteries than were those in spontaneously hypertensive rats (both P <0.05 by analysis of variance) and in Wistar-Kyoto rats (both P <0.05). Similarly, vasorelaxant responses to brain natriuretic peptide were lower both in aortas and in internal carotid arteries of stroke-prone spontaneously hypertensive rats than they were in spontaneously hypertensive rats (both P <0.05) and in Wistar-Kyoto rats (P <0.05). The responses to nitroglycerin in the stroke-prone spontaneously hypertensive rats and spontaneously hypertensive rats fed Japanese-style diet were also similar. Conclusion: The vasorelaxant effects of natriuretic peptides are impaired in stroke-prone spontaneously hypertensive rats. This abnormality could play a role in the pathogenesis of stroke incidence in this hypertensive model.

AB - Background: We have previously shown that a locus on rat chromosome 5, termed STR 2, co-localizes with the genes encoding atrial natriuretic and brain natriuretic peptides, and is closely linked to the development of strokes in rats of a F 2 hybrid cohort obtained by crossing stroke-prone spontaneously hypertensive rats and spontaneously hypertensive rats. We also demonstrated that there are significant differences in vascular functioning that are co-segregated with stroke latency of stroke-prone spontaneously hypertensive rats. Objective: To investigate the vascular responses to natriuretic peptides in the stroke-prone phenotype of spontaneously hypertensive rats. Design and methods: In view of the important vasoactive properties of natriuretic peptides, we tested the vascular responses to 10 -11-10 -9 mol/l atrial natriuretic peptide and to 10 -11-10 -7 mol/l brain natriuretic peptide in isolated rings of aortas and internal carotid arteries obtained from stroke-prone and stroke-resistant spontaneously hypertensive rats. The 6-week-old rats were exposed for 4 weeks either to their regular diet (n = 15 of both strains) or to the stroke-permissive Japanese-style diet (n = 14 of both strains). A group of 14 normotensive, age-matched and sex-matched Wistar-Kyoto rats was also studied. Results: Systolic blood pressures in stroke-prone and stroke-resistant spontaneously hypertensive rats were similar, and were significantly higher than those in Wistar-Kyoto rats. Vascular responses to nitroglycerin, atrial natriuretic peptide, and brain natriuretic peptide in rats of the two hypertensive strains and in Wistar-Kyoto rats fed their regular diet were comparable. In contrast, the vasorelaxant responses to atrial natriuretic peptide in stroke-prone spontaneously hypertensive rats fed Japanese diet were lower both in aortas and in internal carotid arteries than were those in spontaneously hypertensive rats (both P <0.05 by analysis of variance) and in Wistar-Kyoto rats (both P <0.05). Similarly, vasorelaxant responses to brain natriuretic peptide were lower both in aortas and in internal carotid arteries of stroke-prone spontaneously hypertensive rats than they were in spontaneously hypertensive rats (both P <0.05) and in Wistar-Kyoto rats (P <0.05). The responses to nitroglycerin in the stroke-prone spontaneously hypertensive rats and spontaneously hypertensive rats fed Japanese-style diet were also similar. Conclusion: The vasorelaxant effects of natriuretic peptides are impaired in stroke-prone spontaneously hypertensive rats. This abnormality could play a role in the pathogenesis of stroke incidence in this hypertensive model.

KW - Endothelium

KW - Natriuretic peptides

KW - Strokes

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