Impairment of GM-CSF production in myelodysplastic syndromes

G. Visani, G. Zauli, P. Tosi, M. Fogli, A. Cenacchi, C. Finelli, B. Gamberi, E. Ottaviani, S. Manfoi, S. Tura

Research output: Contribution to journalArticlepeer-review

Abstract

In this study we evaluated the production of granulocyte/macrophage-colony stimulating factor (GMCSF), interleukin-6 (IL-6) and tumour necrosis factor-α (TNF-α) by enriched bone marrow (BM) macrophages in 15 patients affected by myelodysplasia and 20 normal BM donors. The presence of GM-CSF, IL-6 and TNF-α in the culture supernatants of BM macrophages was detectable only after stimulation with lipopolysaccharide (LPS), whereas no differences were present in the amount of IL-6 and TNF-α between myelodysplastic patients and normal controls, GM-CSF production appeared eight-fold reduced in BM macrophage culture supernatants from myelodysplastic patients with respect to normal controls. After further experiments, we concluded that the impaired release of GM-CSF by BM macrophages could not be due to a different production kinetic in myelodysplastic patients. Moreover, the number of multipotent (CFU-GEMM), granulocyte/macrophage (CFU-GM) and erythroid (BFU-E) progenitors was significantly impaired in myelodysplastic patients. In conclusion, we demonstrated that the production of GM-CSF by purified adherent cells from MDS patients is markedly impaired in spite of the peripheral blood cytopenia. This selective defect in GM-CSF production, along with an intrinsic defect of haematopoietic progenitor cells, might contribute to the impairment of haematopoiesis always observed in myelodysplastic patients.

Original languageEnglish
Pages (from-to)227-231
Number of pages5
JournalBritish Journal of Haematology
Volume84
Issue number2
Publication statusPublished - 1993

ASJC Scopus subject areas

  • Hematology

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