Impairment of in vitro generation of cytotoxic or t suppressor lymphocytes by Friend leukemia virus infection in mice

E. Garaci, G. Migliorati, T. Jezzi, A. Bartocci, L. Gioia, C. Rinaldi, E. Bonmassar

Research output: Contribution to journalArticle

Abstract

Spleen cells collected from DBA/2 (H-2(d)) mice inoculated with the polycythemic variant of Friend-Leukemia Virus Complex (FLV-P) were tested for T-dependent immune functions, such as the in vitro generation of cytotoxic T lymphocytes (CTL) and of non-specific T suppressor lymphocytes (STL). CTL were generated against H-2(b) splenocytes, and STL were obtained following a 5-day lymphocyte culture without stimulator cells. A progressive and severe impairment of the generation of both CTL and STL was found from 2 weeks onward after infection, being almost totally abolished 3-4 weeks after virus challenge. Suppressor cells (SC) capable of inhibiting CTL generation were detected in FLV-P bearing mice. Suppressor activity was unaffected by anti-Thy 1.2 serum and complement but was removed following iron-magnet depletion or passage through nylon-wool column. Moreover complete recovery of the competence of CTL generation was attained when FLV-P infected splenocytes were passed through nylon-wool column. It is concluded that FLV-P infection depresses T-dependent cytotoxic and suppressor responses in mice, by the appearance of non-T adherent phagocytic cells, capable of impairing CTL generation in vitro.

Original languageEnglish
Pages (from-to)367-373
Number of pages7
JournalInternational Journal of Cancer
Volume28
Issue number3
DOIs
Publication statusPublished - 1981

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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