Impairment of PMP22 transgenic Schwann cells differentiation in culture: Implications for Charcot-Marie-Tooth type 1A disease

Lucilla Nobbio, Tiziana Vigo, Michele Abbruzzese, Giovanni Levi, Claudio Brancolini, Stefano Mantero, Marina Grandis, Luana Benedetti, Gianluigi Mancardi, Angelo Schenone

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Charcot-Marie-Tooth type 1A (CMT1A) is a hereditary demyelinating neuropathy due to an increased genetic dosage of the peripheral myelin protein 22 (PMP22). The mechanisms leading from PMP22 overexpression to impairment of myelination are still unclear. We evaluated expression and processing of PMP22, viability, proliferation, migration, motility and shaping properties, and ability of forming myelin of PMP22 transgenic (PMP22tg) Schwann cells in culture. In basal conditions, PMP22tg Schwann cells, although expressing higher PMP22 levels than control ones, show normal motility, migration and shaping properties. Addition of forskolin to the media induces an additional stimulation of PMP22 expression and results in an impairment of cells migration and motility, and a reduction of cell area and perimeter. Similarly, co-culturing transgenic Schwann cells with neurons causes an altered cells differentiation and an impairment of myelin formation. In conclusion, exposure of PMP22tg Schwann to the axon or to axonal-mimicking stimuli significantly affects the transition of transgenic Schwann cells to the myelinating phenotype.

Original languageEnglish
Pages (from-to)263-273
Number of pages11
JournalNeurobiology of Disease
Volume16
Issue number1
DOIs
Publication statusPublished - Jun 2004

Fingerprint

Myelin Proteins
Charcot-Marie-Tooth Disease
Schwann Cells
Cell Differentiation
Myelin Sheath
Cell Movement
Colforsin
Axons
Tooth
Cell Culture Techniques
Phenotype
Neurons

Keywords

  • Axon
  • CMT1A
  • Dysmyelination
  • Hereditary neuropathy
  • Migration
  • Motility
  • Myelin
  • PMP22
  • Proliferation
  • Schwann cell

ASJC Scopus subject areas

  • Neurology

Cite this

Impairment of PMP22 transgenic Schwann cells differentiation in culture : Implications for Charcot-Marie-Tooth type 1A disease. / Nobbio, Lucilla; Vigo, Tiziana; Abbruzzese, Michele; Levi, Giovanni; Brancolini, Claudio; Mantero, Stefano; Grandis, Marina; Benedetti, Luana; Mancardi, Gianluigi; Schenone, Angelo.

In: Neurobiology of Disease, Vol. 16, No. 1, 06.2004, p. 263-273.

Research output: Contribution to journalArticle

Nobbio, L, Vigo, T, Abbruzzese, M, Levi, G, Brancolini, C, Mantero, S, Grandis, M, Benedetti, L, Mancardi, G & Schenone, A 2004, 'Impairment of PMP22 transgenic Schwann cells differentiation in culture: Implications for Charcot-Marie-Tooth type 1A disease', Neurobiology of Disease, vol. 16, no. 1, pp. 263-273. https://doi.org/10.1016/j.nbd.2004.02.007
Nobbio, Lucilla ; Vigo, Tiziana ; Abbruzzese, Michele ; Levi, Giovanni ; Brancolini, Claudio ; Mantero, Stefano ; Grandis, Marina ; Benedetti, Luana ; Mancardi, Gianluigi ; Schenone, Angelo. / Impairment of PMP22 transgenic Schwann cells differentiation in culture : Implications for Charcot-Marie-Tooth type 1A disease. In: Neurobiology of Disease. 2004 ; Vol. 16, No. 1. pp. 263-273.
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