Implantation of a Poly-L-Lactide GCSF-Functionalized Scaffold in a Model of Chronic Myocardial Infarction

Cristiano Spadaccio, Francesco Nappi, Federico De Marco, Pietro Sedati, Chiara Taffon, Antonio Nenna, Anna Crescenzi, Massimo Chello, Marcella Trombetta, Ivancarmine Gambardella, Alberto Rainer

Research output: Contribution to journalArticlepeer-review

Abstract

A previously developed poly-L-lactide scaffold releasing granulocyte colony-stimulating factor (PLLA/GCSF) was tested in a rabbit chronic model of myocardial infarction (MI) as a ventricular patch. Control groups were constituted by healthy, chronic MI and nonfunctionalized PLLA scaffold. PLLA-based electrospun scaffold efficiently integrated into a chronic infarcted myocardium. Functionalization of the biopolymer with GCSF led to increased fibroblast-like vimentin-positive cellular colonization and reduced inflammatory cell infiltration within the micrometric fiber mesh in comparison to nonfunctionalized scaffold; PLLA/GCSF polymer induced an angiogenetic process with a statistically significant increase in the number of neovessels compared to the nonfunctionalized scaffold; PLLA/GCSF implanted at the infarcted zone induced a reorganization of the ECM architecture leading to connective tissue deposition and scar remodeling. These findings were coupled with a reduction in end-systolic and end-diastolic volumes, indicating a preventive effect of the scaffold on ventricular dilation, and an improvement in cardiac performance.

Original languageEnglish
Pages (from-to)47-65
Number of pages19
JournalJournal of Cardiovascular Translational Research
Volume10
Issue number1
DOIs
Publication statusPublished - Feb 2017

Keywords

  • Animals
  • Chronic Disease
  • Computed Tomography Angiography
  • Disease Models, Animal
  • Drug Carriers
  • Drug Compounding
  • Echocardiography
  • Extracellular Matrix/drug effects
  • Fibroblasts/drug effects
  • Filgrastim/administration & dosage
  • Myocardial Infarction/drug therapy
  • Myocardium/metabolism
  • Neovascularization, Physiologic/drug effects
  • Polyesters/chemistry
  • Rabbits
  • Recovery of Function
  • Time Factors
  • Ventricular Function, Left/drug effects
  • Ventricular Remodeling/drug effects
  • Vimentin/metabolism

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