Abstract
In 2005, the Centro Transfusionale e di Immunomatologia, Dipartimento di Medicina Rigenerativa, an Immunohematology Reference Laboratory in Milan, Italy, instituted a rare donor program to address the transfusion needs of patients with complex immunization to red cell antigens with a rare phenotype. From June 2005 to December 2008, the laboratory used a high-productivity system (Galileo, Immucor, Norcross, GA) for mass-scale antigen screening with profile 1 and 2 antigens for select donors, where 48,715 blood donors were typed with the identification of 6,634 rare blood donors. In April 2009, the laboratory adopted the BeadChip™ platform (BioArray Solutions, Ltd., Warren, NJ) for large-scale DNA typing. The decision to implement was to expand the panel of red blood cell and platelet antigens using the human erythrocyte antigen (HEA) and human platelet antigen (HPA) BeadChip™ formats. As recommended by international guidelines, a validation plan was used to evaluate the sensitivity and specificity of the method. The results of our testing are described in this chapter.
| Original language | English |
|---|---|
| Title of host publication | BeadChip Molecular Immunohematology: Toward Routine Donor and Patient Antigen Profiling by DNA Analysis |
| Publisher | Springer New York |
| Pages | 87-92 |
| Number of pages | 6 |
| ISBN (Print) | 9781441975119 |
| DOIs | |
| Publication status | Published - 2011 |
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Keywords
- Blood donor
- DNA array
- Human erythrocyte antigen
- Human platelet antigen
- Immunohematology reference laboratory
- Phenotype
- Platelet
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Implementation and assessment of high-throughput donor typing at the Milan, Italy, immunohematology reference laboratory. / Revelli, Nicoletta; Paccapelo, Cinzia; Ponzo, Paola; Truglio, Francesca; Sala, Veronica; Poli, Francesca; Marconi, Maurizio; Villa, Maria Antonietta.
BeadChip Molecular Immunohematology: Toward Routine Donor and Patient Antigen Profiling by DNA Analysis. Springer New York, 2011. p. 87-92.Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Implementation and assessment of high-throughput donor typing at the Milan, Italy, immunohematology reference laboratory
AU - Revelli, Nicoletta
AU - Paccapelo, Cinzia
AU - Ponzo, Paola
AU - Truglio, Francesca
AU - Sala, Veronica
AU - Poli, Francesca
AU - Marconi, Maurizio
AU - Villa, Maria Antonietta
PY - 2011
Y1 - 2011
N2 - In 2005, the Centro Transfusionale e di Immunomatologia, Dipartimento di Medicina Rigenerativa, an Immunohematology Reference Laboratory in Milan, Italy, instituted a rare donor program to address the transfusion needs of patients with complex immunization to red cell antigens with a rare phenotype. From June 2005 to December 2008, the laboratory used a high-productivity system (Galileo, Immucor, Norcross, GA) for mass-scale antigen screening with profile 1 and 2 antigens for select donors, where 48,715 blood donors were typed with the identification of 6,634 rare blood donors. In April 2009, the laboratory adopted the BeadChip™ platform (BioArray Solutions, Ltd., Warren, NJ) for large-scale DNA typing. The decision to implement was to expand the panel of red blood cell and platelet antigens using the human erythrocyte antigen (HEA) and human platelet antigen (HPA) BeadChip™ formats. As recommended by international guidelines, a validation plan was used to evaluate the sensitivity and specificity of the method. The results of our testing are described in this chapter.
AB - In 2005, the Centro Transfusionale e di Immunomatologia, Dipartimento di Medicina Rigenerativa, an Immunohematology Reference Laboratory in Milan, Italy, instituted a rare donor program to address the transfusion needs of patients with complex immunization to red cell antigens with a rare phenotype. From June 2005 to December 2008, the laboratory used a high-productivity system (Galileo, Immucor, Norcross, GA) for mass-scale antigen screening with profile 1 and 2 antigens for select donors, where 48,715 blood donors were typed with the identification of 6,634 rare blood donors. In April 2009, the laboratory adopted the BeadChip™ platform (BioArray Solutions, Ltd., Warren, NJ) for large-scale DNA typing. The decision to implement was to expand the panel of red blood cell and platelet antigens using the human erythrocyte antigen (HEA) and human platelet antigen (HPA) BeadChip™ formats. As recommended by international guidelines, a validation plan was used to evaluate the sensitivity and specificity of the method. The results of our testing are described in this chapter.
KW - Blood donor
KW - DNA array
KW - Human erythrocyte antigen
KW - Human platelet antigen
KW - Immunohematology reference laboratory
KW - Phenotype
KW - Platelet
UR - http://www.scopus.com/inward/record.url?scp=84900675114&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84900675114&partnerID=8YFLogxK
U2 - 10.1007/978-1-4419-7512-6_8
DO - 10.1007/978-1-4419-7512-6_8
M3 - Chapter
AN - SCOPUS:84900675114
SN - 9781441975119
SP - 87
EP - 92
BT - BeadChip Molecular Immunohematology: Toward Routine Donor and Patient Antigen Profiling by DNA Analysis
PB - Springer New York
ER -