Implication of limonene and linalyl acetate in cytotoxicity induced by bergamot essential oil in human neuroblastoma cells

Rossella Russo, Antonella Ciociaro, Laura Berliocchi, Maria Gilda Valentina Cassiano, Laura Rombolà, Salvatore Ragusa, Giacinto Bagetta, Fabio Blandini, Maria Tiziana Corasaniti

Research output: Contribution to journalArticle

Abstract

Bergamot (Citrus bergamia, Risso et Poiteau) essential oil (BEO) is a widely used plant extract showing anxiolytic, analgesic and neuroprotective effects in rodents; also, BEO activates multiple death pathways in cancer cells. Despite detailed knowledge of its chemical composition, the constituent/s responsible for these pharmacological activities remain largely unknown. Aim of the present study was to identify the components of BEO implicated in cell death. To this end, limonene, linalyl acetate, linalool, β-terpinene, β-pinene and bergapten were individually tested in human SH-SY5Y neuroblastoma cultures at concentrations comparable with those found in cytotoxic dilutions of BEO. None of the tested compounds elicited cell death. However, significant cytotoxicity was observed when cells were cotreated with limonene and linalyl acetate whereas no other associations were effective. Only cotreatment, but not the single exposure to limonene and linalyl acetate, replicated distinctive morphological and biochemical changes induced by BEO, including caspase-3 activation, PARP cleavage, DNA fragmentation, cell shrinkage, cytoskeletal alterations, together with necrotic and apoptotic cell death. Collectively, our findings suggest a major role for a combined action of these monoterpenes in cancer cell death induced by BEO.

Original languageEnglish
Pages (from-to)48-57
Number of pages10
JournalFitoterapia
Volume89
Issue number1
DOIs
Publication statusPublished - 2013

Fingerprint

Volatile Oils
Neuroblastoma
Cell Death
Monoterpenes
Citrus
Anti-Anxiety Agents
Plant Extracts
Neuroprotective Agents
DNA Fragmentation
Caspase 3
Analgesics
Rodentia
Neoplasms
Pharmacology
linalyl acetate
limonene
bergamot oil

Keywords

  • Bergamot essential oil
  • Limonene
  • Linalool
  • Linalyl acetate
  • Monoterpenes
  • Natural products

ASJC Scopus subject areas

  • Pharmacology
  • Drug Discovery

Cite this

Russo, R., Ciociaro, A., Berliocchi, L., Valentina Cassiano, M. G., Rombolà, L., Ragusa, S., ... Corasaniti, M. T. (2013). Implication of limonene and linalyl acetate in cytotoxicity induced by bergamot essential oil in human neuroblastoma cells. Fitoterapia, 89(1), 48-57. https://doi.org/10.1016/j.fitote.2013.05.014

Implication of limonene and linalyl acetate in cytotoxicity induced by bergamot essential oil in human neuroblastoma cells. / Russo, Rossella; Ciociaro, Antonella; Berliocchi, Laura; Valentina Cassiano, Maria Gilda; Rombolà, Laura; Ragusa, Salvatore; Bagetta, Giacinto; Blandini, Fabio; Corasaniti, Maria Tiziana.

In: Fitoterapia, Vol. 89, No. 1, 2013, p. 48-57.

Research output: Contribution to journalArticle

Russo, R, Ciociaro, A, Berliocchi, L, Valentina Cassiano, MG, Rombolà, L, Ragusa, S, Bagetta, G, Blandini, F & Corasaniti, MT 2013, 'Implication of limonene and linalyl acetate in cytotoxicity induced by bergamot essential oil in human neuroblastoma cells', Fitoterapia, vol. 89, no. 1, pp. 48-57. https://doi.org/10.1016/j.fitote.2013.05.014
Russo, Rossella ; Ciociaro, Antonella ; Berliocchi, Laura ; Valentina Cassiano, Maria Gilda ; Rombolà, Laura ; Ragusa, Salvatore ; Bagetta, Giacinto ; Blandini, Fabio ; Corasaniti, Maria Tiziana. / Implication of limonene and linalyl acetate in cytotoxicity induced by bergamot essential oil in human neuroblastoma cells. In: Fitoterapia. 2013 ; Vol. 89, No. 1. pp. 48-57.
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