Implication of OTX2 in pigment epithelium determination and neural retina differentiation

Paola Bovolenta, Antonello Mallamaci, Paola Briata, Giorgio Corte, Edoardo Boncinelli

Research output: Contribution to journalArticle

122 Citations (Scopus)

Abstract

The expression pattern of Otx2, a homeobox-containing gene, was analyzed from the beginning of eye morphogenesis until neural retina differentiation in chick embryos. Early on, Otx2 expression was diffuse throughout the optic vesicles but became restricted to their dorsal part when the vesicles contacted the surface ectoderm. As the optic cup forms, Otx2 was expressed only in the outer layer, which gives rise to the pigment epithelium. This early Otx2 expression pattern was complementary to that of PAX2, which localizes to the ventral half of the developing eye and optic stalk. Otx2 expression was always observed in the pigment epithelium at all stages analyzed but was extended to scattered cells located in the central portion of the neural retina around stage 22. The number of cells expressing Otx2 transcripts increased with time, following a central to peripheral gradient. Bromodeoxyuridine labeling in combination with immunohistochemistry with anti-OTX2 antiserum and different cell-specific markers were used to determine that OTX2-positive cells are postmitotic neuroblasts undergoing differentiation into several, if not all, of the distinct cell types present in the chick retina. These data indicate that Otx2 might have a double role in eye development. First, it might be necessary for the early specification and subsequent functioning of the pigment epithelium. Later, OTX2 expression might be involved in retina neurogenesis, defining a differentiation feature common to the distinct retinal cell classes.

Original languageEnglish
Pages (from-to)4243-4252
Number of pages10
JournalJournal of Neuroscience
Volume17
Issue number11
Publication statusPublished - 1997

Fingerprint

Retina
Epithelium
Ectoderm
Homeobox Genes
Neurogenesis
Chick Embryo
Bromodeoxyuridine
Morphogenesis
Immune Sera
Cell Count
Immunohistochemistry

Keywords

  • chick
  • neurogenesis
  • optic cup
  • PAX2
  • pigment epithelium
  • postmitotic neuroblast
  • retinal ganglion cell

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Bovolenta, P., Mallamaci, A., Briata, P., Corte, G., & Boncinelli, E. (1997). Implication of OTX2 in pigment epithelium determination and neural retina differentiation. Journal of Neuroscience, 17(11), 4243-4252.

Implication of OTX2 in pigment epithelium determination and neural retina differentiation. / Bovolenta, Paola; Mallamaci, Antonello; Briata, Paola; Corte, Giorgio; Boncinelli, Edoardo.

In: Journal of Neuroscience, Vol. 17, No. 11, 1997, p. 4243-4252.

Research output: Contribution to journalArticle

Bovolenta, P, Mallamaci, A, Briata, P, Corte, G & Boncinelli, E 1997, 'Implication of OTX2 in pigment epithelium determination and neural retina differentiation', Journal of Neuroscience, vol. 17, no. 11, pp. 4243-4252.
Bovolenta, Paola ; Mallamaci, Antonello ; Briata, Paola ; Corte, Giorgio ; Boncinelli, Edoardo. / Implication of OTX2 in pigment epithelium determination and neural retina differentiation. In: Journal of Neuroscience. 1997 ; Vol. 17, No. 11. pp. 4243-4252.
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