Improved collection of mobilized CD34+ hematopoietic progenitor cells by a novel automated leukapheresis system

F. Ravagnani, S. Siena, S. De Reys, M. Di Nicola, P. Notti, R. Giardini, M. Bregni, P. Matteucci, A. M. Gianni, G. Pellegris

Research output: Contribution to journalArticlepeer-review

Abstract

BACKGROUND: For simplification of blood cell transplantation, an automated apheresis system that exploits a dual-stage channel device for mononuclear cell (MNC) collection (AutoPBSC, designed for the COBE Spectra) was studied. STUDY DESIGN AND METHODS: The automated default software (AutoPBSC-Default) and three software modifications of the harvest frequency during leukapheresis, referred to as AutoPBSC-1.25, AutoPBSC-1.75, and AutoPBSC-2.75, were evaluated in comparison with the semiautomated Version 4.7 (V4.7) apheresis system in 119 leukapheresis procedures performed in 90 cancer patients treated with chemotherapy plus granulocyte-colony-stimulating factor. CD34+ cell and platelet collection efficiency (CE); volume and cell composition of the leukapheresis components; and patient platelet and red cell (RBC) loss during leukapheresis were measured. RESULTS: The majority of collection measures evaluated with the AutoPBSC compared favorably to those obtained with the V4.7. CD34+ cell CE increased from 55 percent with V4.7 to 68 percent with the AutoPBSC-Default (p = 0.05). The AutoPBSC provided lower platelet contamination in the collected component (1.18 x 1011 vs. 2.26 x 1011 with the V4.7; p11 vs. 1.04 x 1011 with the V4.7; p

Original languageEnglish
Pages (from-to)48-55
Number of pages8
JournalTransfusion
Volume39
Issue number1
Publication statusPublished - 1999

ASJC Scopus subject areas

  • Hematology
  • Immunology

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