Abstract
Ferene(TM) (Diagnostic Chemicals LTD, Canada) is used as the chromogenic agent in an improved direct method for determining serum iron. The colour reagent contains hydroxylamine, a reducing agent stable in solution, and a protein solubilizer to avoid deproteinization. The interference of serum copper is eliminated by complexation with thiourea. Reliability of the method has been established by a separate study of the reaction parameters, replicate analysis and recovery experiments. The results obtained by the proposed method correlate well with results of atomic absorption spectroscopy. A fully automated application is also proposed using a centrifugal analyzer with starter dispenser.
| Original language | English |
|---|---|
| Pages (from-to) | 6-10 |
| Number of pages | 5 |
| Journal | Haematologica |
| Volume | 70 |
| Issue number | 1 |
| Publication status | Published - 1985 |
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ASJC Scopus subject areas
- Hematology
Cite this
Improved direct determination of serum iron. / Giuliani, F.; De Luca, U.; Melzi d'Eril, G. V.; Moratti, R.
In: Haematologica, Vol. 70, No. 1, 1985, p. 6-10.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Improved direct determination of serum iron
AU - Giuliani, F.
AU - De Luca, U.
AU - Melzi d'Eril, G. V.
AU - Moratti, R.
PY - 1985
Y1 - 1985
N2 - Ferene(TM) (Diagnostic Chemicals LTD, Canada) is used as the chromogenic agent in an improved direct method for determining serum iron. The colour reagent contains hydroxylamine, a reducing agent stable in solution, and a protein solubilizer to avoid deproteinization. The interference of serum copper is eliminated by complexation with thiourea. Reliability of the method has been established by a separate study of the reaction parameters, replicate analysis and recovery experiments. The results obtained by the proposed method correlate well with results of atomic absorption spectroscopy. A fully automated application is also proposed using a centrifugal analyzer with starter dispenser.
AB - Ferene(TM) (Diagnostic Chemicals LTD, Canada) is used as the chromogenic agent in an improved direct method for determining serum iron. The colour reagent contains hydroxylamine, a reducing agent stable in solution, and a protein solubilizer to avoid deproteinization. The interference of serum copper is eliminated by complexation with thiourea. Reliability of the method has been established by a separate study of the reaction parameters, replicate analysis and recovery experiments. The results obtained by the proposed method correlate well with results of atomic absorption spectroscopy. A fully automated application is also proposed using a centrifugal analyzer with starter dispenser.
UR - http://www.scopus.com/inward/record.url?scp=0022259767&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0022259767&partnerID=8YFLogxK
M3 - Article
VL - 70
SP - 6
EP - 10
JO - Haematologica
JF - Haematologica
SN - 0390-6078
IS - 1
ER -