Improved direct determination of serum iron

F. Giuliani, U. De Luca, G. V. Melzi d'Eril, R. Moratti

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Ferene(TM) (Diagnostic Chemicals LTD, Canada) is used as the chromogenic agent in an improved direct method for determining serum iron. The colour reagent contains hydroxylamine, a reducing agent stable in solution, and a protein solubilizer to avoid deproteinization. The interference of serum copper is eliminated by complexation with thiourea. Reliability of the method has been established by a separate study of the reaction parameters, replicate analysis and recovery experiments. The results obtained by the proposed method correlate well with results of atomic absorption spectroscopy. A fully automated application is also proposed using a centrifugal analyzer with starter dispenser.

Original languageEnglish
Pages (from-to)6-10
Number of pages5
JournalHaematologica
Volume70
Issue number1
Publication statusPublished - 1985

Fingerprint

Iron
Serum
Thiourea
Hydroxylamine
Reducing Agents
Canada
Copper
Spectrum Analysis
Color
Proteins
Ferene-S

ASJC Scopus subject areas

  • Hematology

Cite this

Giuliani, F., De Luca, U., Melzi d'Eril, G. V., & Moratti, R. (1985). Improved direct determination of serum iron. Haematologica, 70(1), 6-10.

Improved direct determination of serum iron. / Giuliani, F.; De Luca, U.; Melzi d'Eril, G. V.; Moratti, R.

In: Haematologica, Vol. 70, No. 1, 1985, p. 6-10.

Research output: Contribution to journalArticle

Giuliani, F, De Luca, U, Melzi d'Eril, GV & Moratti, R 1985, 'Improved direct determination of serum iron', Haematologica, vol. 70, no. 1, pp. 6-10.
Giuliani F, De Luca U, Melzi d'Eril GV, Moratti R. Improved direct determination of serum iron. Haematologica. 1985;70(1):6-10.
Giuliani, F. ; De Luca, U. ; Melzi d'Eril, G. V. ; Moratti, R. / Improved direct determination of serum iron. In: Haematologica. 1985 ; Vol. 70, No. 1. pp. 6-10.
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