Abstract
Ferene(TM) (Diagnostic Chemicals LTD, Canada) is used as the chromogenic agent in an improved direct method for determining serum iron. The colour reagent contains hydroxylamine, a reducing agent stable in solution, and a protein solubilizer to avoid deproteinization. The interference of serum copper is eliminated by complexation with thiourea. Reliability of the method has been established by a separate study of the reaction parameters, replicate analysis and recovery experiments. The results obtained by the proposed method correlate well with results of atomic absorption spectroscopy. A fully automated application is also proposed using a centrifugal analyzer with starter dispenser.
| Original language | English |
|---|---|
| Pages (from-to) | 6-10 |
| Number of pages | 5 |
| Journal | Haematologica |
| Volume | 70 |
| Issue number | 1 |
| Publication status | Published - 1985 |
ASJC Scopus subject areas
- Hematology
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Giuliani, F., De Luca, U., Melzi d'Eril, G. V., & Moratti, R. (1985). Improved direct determination of serum iron. Haematologica, 70(1), 6-10.