Improved direct determination of serum iron

F. Giuliani, U. De Luca, G. V. Melzi d'Eril, R. Moratti

Research output: Contribution to journalArticle

Abstract

Ferene(TM) (Diagnostic Chemicals LTD, Canada) is used as the chromogenic agent in an improved direct method for determining serum iron. The colour reagent contains hydroxylamine, a reducing agent stable in solution, and a protein solubilizer to avoid deproteinization. The interference of serum copper is eliminated by complexation with thiourea. Reliability of the method has been established by a separate study of the reaction parameters, replicate analysis and recovery experiments. The results obtained by the proposed method correlate well with results of atomic absorption spectroscopy. A fully automated application is also proposed using a centrifugal analyzer with starter dispenser.

Original languageEnglish
Pages (from-to)6-10
Number of pages5
JournalHaematologica
Volume70
Issue number1
Publication statusPublished - 1985

ASJC Scopus subject areas

  • Hematology

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    Giuliani, F., De Luca, U., Melzi d'Eril, G. V., & Moratti, R. (1985). Improved direct determination of serum iron. Haematologica, 70(1), 6-10.