Improved direct specific determination of serum iron and total iron-binding capacity

F. Ceriotti, G. Ceriotti

Research output: Contribution to journalArticle

Abstract

Serum iron is released from transferrin and reduced at pH 1.7 by treating serum with a 10 g/L ascorbic acid solution in 0.1 mol/L HCl. When ferrozine is added to this reagent, it forms a complex with iron that is as intensely colored as at higher pH values, and under these conditions no turbidity is produced. The second major interference, that from copper, is eliminated by adding 1 g of thiosemicarbazide per liter, which at a low pH forms a stable, uncolored complex with copper without affecting the reaction of ferrozine with iron.

Original languageEnglish
Pages (from-to)327-331
Number of pages5
JournalClinical Chemistry
Volume26
Issue number2
Publication statusPublished - 1980

ASJC Scopus subject areas

  • Clinical Biochemistry

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