Improved function of circulating angiogenic cells is evident in type 1 diabetic islet-transplanted patients

A. Petrelli, A. Maestroni, G. P. Fadini, D. Belloni, M. Venturini, M. Albiero, S. Kleffel, B. G. Mfarrej, A. Del Maschio, P. Maffi, A. Avogaro, E. Ferrero, G. Zerbini, A. Secchi, P. Fiorina

Research output: Contribution to journalArticlepeer-review


Circulating angiogenic cells (CACs) are vascular-committed bone marrow-derived cells that are dysfunctional in type 1 diabetes (T1D). Here we studied whether restoration of normoglycemia following islet transplantation is associated with better CAC function. We carried out a cross-sectional study of 18 T1D patients, 14 insulin-independent islet-transplanted patients (ITA) and 14 healthy controls (C) evaluating in vivo and in vitro CACs viability and function. We found that the percentage of CACs in vivo did not differ among the three groups while the number of CAC colonies obtained from T1D, but not from ITA, was reduced compared to C (C = 7.3 ± 1.9, T1D = 0.9 ± 0.4 and ITA = 4.7 ± 1.9; p <0.05 T1D vs. all). In vitro CAC migration/differentiation were similar, while in vivo an improved angiogenic ability of ITA compared to T1D was shown (capillary density: C = 93.5 ± 22.1, T1D = 19.2 ± 2.8 and ITA = 44.0 ± 10.5, p <0.05 T1D vs. all). Increased apoptosis and lesser IL-8 secretion were evident in CACs obtained from T1D compared to C and ITA. in vitro addition of anti-hIL-8 reduced the number of colonies obtained from C. Finally, T1D, but not ITA, had a lower endothelial-dependent dilatation (EDD) compared with C. These data suggest that CAC function is altered in T1D and may be improved after islet transplantation.

Original languageEnglish
Pages (from-to)2690-2700
Number of pages11
JournalAmerican Journal of Transplantation
Issue number12
Publication statusPublished - Dec 2010


  • IL-8
  • islet transplantation
  • laboratory science
  • original article
  • stem cells
  • type 1 diabetes

ASJC Scopus subject areas

  • Transplantation
  • Immunology and Allergy
  • Pharmacology (medical)


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