Improved microfluorometric DNA determination in biological material using 33258 Hoechst

C. F. Cesarone; C. Bolognesi; L. Santi

doi:10.1016/0003-2697(79)90131-3

Improved microfluorometric DNA determination in biological material using 33258 Hoechst

C. F. Cesarone, C. Bolognesi, L. Santi

A.O.U. San Martino - IST, Istituto Nazionale Ricerca sul Cancro (GENOVA)

Research output: Contribution to journal › Article › peer-review

Abstract

A simple and rapid microfluorometric method is described for the determination of DNA in submicrogram quantities using 33258 Hoechst fluorochrome. A high degree of reproducibility was obtained using calf thymus and phage DNA, mouse liver chromatin, and HeLa cells homogenate preparations. None or very little interference by the routinely used preparation reagents or by the cellular components was found. Compared to other commonly used procedures this innovative and versatile technique can be conveniently applied to DNA microdetermination for the high sensibility/reproducibility ratio and can also be used without the need of previous purification steps.

Original language	English
Pages (from-to)	188-197
Number of pages	10
Journal	Analytical Biochemistry
Volume	100
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(79)90131-3
Publication status	Published - Nov 15 1979

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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10.1016/0003-2697(79)90131-3

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N2 - A simple and rapid microfluorometric method is described for the determination of DNA in submicrogram quantities using 33258 Hoechst fluorochrome. A high degree of reproducibility was obtained using calf thymus and phage DNA, mouse liver chromatin, and HeLa cells homogenate preparations. None or very little interference by the routinely used preparation reagents or by the cellular components was found. Compared to other commonly used procedures this innovative and versatile technique can be conveniently applied to DNA microdetermination for the high sensibility/reproducibility ratio and can also be used without the need of previous purification steps.

AB - A simple and rapid microfluorometric method is described for the determination of DNA in submicrogram quantities using 33258 Hoechst fluorochrome. A high degree of reproducibility was obtained using calf thymus and phage DNA, mouse liver chromatin, and HeLa cells homogenate preparations. None or very little interference by the routinely used preparation reagents or by the cellular components was found. Compared to other commonly used procedures this innovative and versatile technique can be conveniently applied to DNA microdetermination for the high sensibility/reproducibility ratio and can also be used without the need of previous purification steps.

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Improved microfluorometric DNA determination in biological material using 33258 Hoechst

Abstract

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