Abstract
A simple and rapid microfluorometric method is described for the determination of DNA in submicrogram quantities using 33258 Hoechst fluorochrome. A high degree of reproducibility was obtained using calf thymus and phage DNA, mouse liver chromatin, and HeLa cells homogenate preparations. None or very little interference by the routinely used preparation reagents or by the cellular components was found. Compared to other commonly used procedures this innovative and versatile technique can be conveniently applied to DNA microdetermination for the high sensibility/reproducibility ratio and can also be used without the need of previous purification steps.
| Original language | English |
|---|---|
| Pages (from-to) | 188-197 |
| Number of pages | 10 |
| Journal | Analytical Biochemistry |
| Volume | 100 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Nov 15 1979 |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
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Improved microfluorometric DNA determination in biological material using 33258 Hoechst. / Cesarone, C. F.; Bolognesi, C.; Santi, L.
In: Analytical Biochemistry, Vol. 100, No. 1, 15.11.1979, p. 188-197.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Improved microfluorometric DNA determination in biological material using 33258 Hoechst
AU - Cesarone, C. F.
AU - Bolognesi, C.
AU - Santi, L.
PY - 1979/11/15
Y1 - 1979/11/15
N2 - A simple and rapid microfluorometric method is described for the determination of DNA in submicrogram quantities using 33258 Hoechst fluorochrome. A high degree of reproducibility was obtained using calf thymus and phage DNA, mouse liver chromatin, and HeLa cells homogenate preparations. None or very little interference by the routinely used preparation reagents or by the cellular components was found. Compared to other commonly used procedures this innovative and versatile technique can be conveniently applied to DNA microdetermination for the high sensibility/reproducibility ratio and can also be used without the need of previous purification steps.
AB - A simple and rapid microfluorometric method is described for the determination of DNA in submicrogram quantities using 33258 Hoechst fluorochrome. A high degree of reproducibility was obtained using calf thymus and phage DNA, mouse liver chromatin, and HeLa cells homogenate preparations. None or very little interference by the routinely used preparation reagents or by the cellular components was found. Compared to other commonly used procedures this innovative and versatile technique can be conveniently applied to DNA microdetermination for the high sensibility/reproducibility ratio and can also be used without the need of previous purification steps.
UR - http://www.scopus.com/inward/record.url?scp=0018568031&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0018568031&partnerID=8YFLogxK
U2 - 10.1016/0003-2697(79)90131-3
DO - 10.1016/0003-2697(79)90131-3
M3 - Article
C2 - 94515
AN - SCOPUS:0018568031
VL - 100
SP - 188
EP - 197
JO - Analytical Biochemistry
JF - Analytical Biochemistry
SN - 0003-2697
IS - 1
ER -