Improved microfluorometric DNA determination in biological material using 33258 Hoechst

C. F. Cesarone, C. Bolognesi, L. Santi

Research output: Contribution to journalArticle

735 Citations (Scopus)

Abstract

A simple and rapid microfluorometric method is described for the determination of DNA in submicrogram quantities using 33258 Hoechst fluorochrome. A high degree of reproducibility was obtained using calf thymus and phage DNA, mouse liver chromatin, and HeLa cells homogenate preparations. None or very little interference by the routinely used preparation reagents or by the cellular components was found. Compared to other commonly used procedures this innovative and versatile technique can be conveniently applied to DNA microdetermination for the high sensibility/reproducibility ratio and can also be used without the need of previous purification steps.

Original languageEnglish
Pages (from-to)188-197
Number of pages10
JournalAnalytical Biochemistry
Volume100
Issue number1
DOIs
Publication statusPublished - Nov 15 1979

Fingerprint

Bisbenzimidazole
Biological materials
DNA
Fluorescent Dyes
HeLa Cells
Bacteriophages
Chromatin
Thymus
Liver
Purification
calf thymus DNA

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Improved microfluorometric DNA determination in biological material using 33258 Hoechst. / Cesarone, C. F.; Bolognesi, C.; Santi, L.

In: Analytical Biochemistry, Vol. 100, No. 1, 15.11.1979, p. 188-197.

Research output: Contribution to journalArticle

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