Improvement of diaphragmatic performance through orthotopic application of decellularized extracellular matrix patch

M. Piccoli; L. Urbani; M. E. Alvarez-Fallas; C. Franzin; A. Dedja; E. Bertin; G. Zuccolotto; A. Rosato; P. Pavan; N. Elvassore; P. De Coppi; M. Pozzobon

doi:10.1016/j.biomaterials.2015.10.005

Improvement of diaphragmatic performance through orthotopic application of decellularized extracellular matrix patch

M. Piccoli, L. Urbani, M. E. Alvarez-Fallas, C. Franzin, A. Dedja, E. Bertin, G. Zuccolotto, A. Rosato, P. Pavan, N. Elvassore, P. De Coppi, M. Pozzobon

Istituto Oncologico Veneto

Research output: Contribution to journal › Article › peer-review

Abstract

Muscle tissue engineering can provide support to large congenital skeletal muscle defects using scaffolds able to allow cell migration, proliferation and differentiation. Acellular extracellular matrix (ECM) scaffold can generate a positive inflammatory response through the activation of anti-inflammatory T-cell populations and M2 polarized macrophages that together lead to a local pro-regenerative environment. This immunoregulatory effect is maintained when acellular matrices are transplanted in a xenogeneic setting, but it remains unclear whether it can be therapeutic in a model of muscle diseases. We demonstrated here for the first time that orthotopic transplantation of a decellularized diaphragmatic muscle from wild animals promoted tissue functional recovery in an established atrophic mouse model. In particular, ECM supported a local immunoresponse activating a pro-regenerative environment and stimulating host muscle progenitor cell activation and migration. These results indicate that acellular scaffolds may represent a suitable regenerative medicine option for improving performance of diseased muscles.

Original language	English
Pages (from-to)	245-255
Number of pages	11
Journal	Biomaterials
Volume	74
DOIs	https://doi.org/10.1016/j.biomaterials.2015.10.005
Publication status	Published - Jan 1 2016

Keywords

Decellularization
Extracellular matrix
Regenerative medicine
Skeletal muscle

ASJC Scopus subject areas

Biomaterials
Bioengineering
Ceramics and Composites
Mechanics of Materials
Biophysics

Access to Document

10.1016/j.biomaterials.2015.10.005

Cite this

Piccoli, M., Urbani, L., Alvarez-Fallas, M. E., Franzin, C., Dedja, A., Bertin, E., Zuccolotto, G., Rosato, A., Pavan, P., Elvassore, N., De Coppi, P., & Pozzobon, M. (2016). Improvement of diaphragmatic performance through orthotopic application of decellularized extracellular matrix patch. Biomaterials, 74, 245-255. https://doi.org/10.1016/j.biomaterials.2015.10.005

Improvement of diaphragmatic performance through orthotopic application of decellularized extracellular matrix patch. / Piccoli, M.; Urbani, L.; Alvarez-Fallas, M. E.; Franzin, C.; Dedja, A.; Bertin, E.; Zuccolotto, G.; Rosato, A.; Pavan, P.; Elvassore, N.; De Coppi, P.; Pozzobon, M.

In: Biomaterials, Vol. 74, 01.01.2016, p. 245-255.

Research output: Contribution to journal › Article › peer-review

@article{da0a0365aaaa4e339ea6c524f519f61e,

title = "Improvement of diaphragmatic performance through orthotopic application of decellularized extracellular matrix patch",

abstract = "Muscle tissue engineering can provide support to large congenital skeletal muscle defects using scaffolds able to allow cell migration, proliferation and differentiation. Acellular extracellular matrix (ECM) scaffold can generate a positive inflammatory response through the activation of anti-inflammatory T-cell populations and M2 polarized macrophages that together lead to a local pro-regenerative environment. This immunoregulatory effect is maintained when acellular matrices are transplanted in a xenogeneic setting, but it remains unclear whether it can be therapeutic in a model of muscle diseases. We demonstrated here for the first time that orthotopic transplantation of a decellularized diaphragmatic muscle from wild animals promoted tissue functional recovery in an established atrophic mouse model. In particular, ECM supported a local immunoresponse activating a pro-regenerative environment and stimulating host muscle progenitor cell activation and migration. These results indicate that acellular scaffolds may represent a suitable regenerative medicine option for improving performance of diseased muscles.",

keywords = "Decellularization, Extracellular matrix, Regenerative medicine, Skeletal muscle",

author = "M. Piccoli and L. Urbani and Alvarez-Fallas, {M. E.} and C. Franzin and A. Dedja and E. Bertin and G. Zuccolotto and A. Rosato and P. Pavan and N. Elvassore and {De Coppi}, P. and M. Pozzobon",

year = "2016",

month = jan,

day = "1",

doi = "10.1016/j.biomaterials.2015.10.005",

language = "English",

volume = "74",

pages = "245--255",

journal = "Biomaterials",

issn = "0142-9612",

publisher = "Elsevier BV",

}

TY - JOUR

T1 - Improvement of diaphragmatic performance through orthotopic application of decellularized extracellular matrix patch

AU - Piccoli, M.

AU - Urbani, L.

AU - Alvarez-Fallas, M. E.

AU - Franzin, C.

AU - Dedja, A.

AU - Bertin, E.

AU - Zuccolotto, G.

AU - Rosato, A.

AU - Pavan, P.

AU - Elvassore, N.

AU - De Coppi, P.

AU - Pozzobon, M.

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Muscle tissue engineering can provide support to large congenital skeletal muscle defects using scaffolds able to allow cell migration, proliferation and differentiation. Acellular extracellular matrix (ECM) scaffold can generate a positive inflammatory response through the activation of anti-inflammatory T-cell populations and M2 polarized macrophages that together lead to a local pro-regenerative environment. This immunoregulatory effect is maintained when acellular matrices are transplanted in a xenogeneic setting, but it remains unclear whether it can be therapeutic in a model of muscle diseases. We demonstrated here for the first time that orthotopic transplantation of a decellularized diaphragmatic muscle from wild animals promoted tissue functional recovery in an established atrophic mouse model. In particular, ECM supported a local immunoresponse activating a pro-regenerative environment and stimulating host muscle progenitor cell activation and migration. These results indicate that acellular scaffolds may represent a suitable regenerative medicine option for improving performance of diseased muscles.

AB - Muscle tissue engineering can provide support to large congenital skeletal muscle defects using scaffolds able to allow cell migration, proliferation and differentiation. Acellular extracellular matrix (ECM) scaffold can generate a positive inflammatory response through the activation of anti-inflammatory T-cell populations and M2 polarized macrophages that together lead to a local pro-regenerative environment. This immunoregulatory effect is maintained when acellular matrices are transplanted in a xenogeneic setting, but it remains unclear whether it can be therapeutic in a model of muscle diseases. We demonstrated here for the first time that orthotopic transplantation of a decellularized diaphragmatic muscle from wild animals promoted tissue functional recovery in an established atrophic mouse model. In particular, ECM supported a local immunoresponse activating a pro-regenerative environment and stimulating host muscle progenitor cell activation and migration. These results indicate that acellular scaffolds may represent a suitable regenerative medicine option for improving performance of diseased muscles.

KW - Decellularization

KW - Extracellular matrix

KW - Regenerative medicine

KW - Skeletal muscle

UR - http://www.scopus.com/inward/record.url?scp=84945156159&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84945156159&partnerID=8YFLogxK

U2 - 10.1016/j.biomaterials.2015.10.005

DO - 10.1016/j.biomaterials.2015.10.005

M3 - Article

AN - SCOPUS:84945156159

VL - 74

SP - 245

EP - 255

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

ER -

Improvement of diaphragmatic performance through orthotopic application of decellularized extracellular matrix patch

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this