Improvements in megakaryocyte purification

L. Contino, G. Vandelli, P. Lanzarini, G. Sitar

Research output: Contribution to journalArticle

Abstract

An improved two step procedure to purify megakaryocytes (MK) is proposed. Guinea Pig bone marrow particles are incubated with collagenase to obtain a single cell suspension which is further processed by buoyant density gradient centrifugation and by velocity sedimentation using a modified sedimentation chamber. An high degree of MK purification is achieved. Purified MK were investigated by Scanning Electron Microscopy (SEM). Our observations provide new insight on the surface structure of these cells.

Original languageEnglish
Pages (from-to)607-614
Number of pages8
JournalHaematologica
Volume68
Issue number5
Publication statusPublished - 1983

ASJC Scopus subject areas

  • Hematology

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    Contino, L., Vandelli, G., Lanzarini, P., & Sitar, G. (1983). Improvements in megakaryocyte purification. Haematologica, 68(5), 607-614.