Improvements of Western blotting to detect monoclonal antibodies

Marco Bestagno, Antonella Cerino, Silvano Riva, Giulia C B Astaldi Ricotti

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

A comparison of the effects of different factors on the sensitivity of Western blotting technique to detect monoclonal antibodies is described. The major improvements were obtained by: A) renaturating the antigen in the gel before transferring it in carbonate buffer at pH 10 onto nitrocellulose and B) using alkaline-phosphatase-conjugated second antibody instead of peroxidase-conjugated second antibody.

Original languageEnglish
Pages (from-to)1509-1514
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume146
Issue number3
DOIs
Publication statusPublished - Aug 14 1987

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Western Blotting
Monoclonal Antibodies
Collodion
Antibodies
Carbonates
Peroxidase
Alkaline Phosphatase
Buffers
Gels
Antigens

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Improvements of Western blotting to detect monoclonal antibodies. / Bestagno, Marco; Cerino, Antonella; Riva, Silvano; Astaldi Ricotti, Giulia C B.

In: Biochemical and Biophysical Research Communications, Vol. 146, No. 3, 14.08.1987, p. 1509-1514.

Research output: Contribution to journalArticle

Bestagno, Marco ; Cerino, Antonella ; Riva, Silvano ; Astaldi Ricotti, Giulia C B. / Improvements of Western blotting to detect monoclonal antibodies. In: Biochemical and Biophysical Research Communications. 1987 ; Vol. 146, No. 3. pp. 1509-1514.
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