Abstract
A comparison of the effects of different factors on the sensitivity of Western blotting technique to detect monoclonal antibodies is described. The major improvements were obtained by: A) renaturating the antigen in the gel before transferring it in carbonate buffer at pH 10 onto nitrocellulose and B) using alkaline-phosphatase-conjugated second antibody instead of peroxidase-conjugated second antibody.
| Original language | English |
|---|---|
| Pages (from-to) | 1509-1514 |
| Number of pages | 6 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 146 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Aug 14 1987 |
Fingerprint
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Improvements of Western blotting to detect monoclonal antibodies. / Bestagno, Marco; Cerino, Antonella; Riva, Silvano; Astaldi Ricotti, Giulia C B.
In: Biochemical and Biophysical Research Communications, Vol. 146, No. 3, 14.08.1987, p. 1509-1514.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Improvements of Western blotting to detect monoclonal antibodies
AU - Bestagno, Marco
AU - Cerino, Antonella
AU - Riva, Silvano
AU - Astaldi Ricotti, Giulia C B
PY - 1987/8/14
Y1 - 1987/8/14
N2 - A comparison of the effects of different factors on the sensitivity of Western blotting technique to detect monoclonal antibodies is described. The major improvements were obtained by: A) renaturating the antigen in the gel before transferring it in carbonate buffer at pH 10 onto nitrocellulose and B) using alkaline-phosphatase-conjugated second antibody instead of peroxidase-conjugated second antibody.
AB - A comparison of the effects of different factors on the sensitivity of Western blotting technique to detect monoclonal antibodies is described. The major improvements were obtained by: A) renaturating the antigen in the gel before transferring it in carbonate buffer at pH 10 onto nitrocellulose and B) using alkaline-phosphatase-conjugated second antibody instead of peroxidase-conjugated second antibody.
UR - http://www.scopus.com/inward/record.url?scp=0023653274&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023653274&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(87)90820-5
DO - 10.1016/0006-291X(87)90820-5
M3 - Article
VL - 146
SP - 1509
EP - 1514
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -