TY - JOUR
T1 - Improvements of Western blotting to detect monoclonal antibodies
AU - Bestagno, Marco
AU - Cerino, Antonella
AU - Riva, Silvano
AU - Astaldi Ricotti, Giulia C B
PY - 1987/8/14
Y1 - 1987/8/14
N2 - A comparison of the effects of different factors on the sensitivity of Western blotting technique to detect monoclonal antibodies is described. The major improvements were obtained by: A) renaturating the antigen in the gel before transferring it in carbonate buffer at pH 10 onto nitrocellulose and B) using alkaline-phosphatase-conjugated second antibody instead of peroxidase-conjugated second antibody.
AB - A comparison of the effects of different factors on the sensitivity of Western blotting technique to detect monoclonal antibodies is described. The major improvements were obtained by: A) renaturating the antigen in the gel before transferring it in carbonate buffer at pH 10 onto nitrocellulose and B) using alkaline-phosphatase-conjugated second antibody instead of peroxidase-conjugated second antibody.
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U2 - 10.1016/0006-291X(87)90820-5
DO - 10.1016/0006-291X(87)90820-5
M3 - Article
C2 - 3304295
AN - SCOPUS:0023653274
VL - 146
SP - 1509
EP - 1514
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -