Improvements of Western blotting to detect monoclonal antibodies

Marco Bestagno; Antonella Cerino; Silvano Riva; Giulia C B Astaldi Ricotti

doi:10.1016/0006-291X(87)90820-5

Improvements of Western blotting to detect monoclonal antibodies

Marco Bestagno, Antonella Cerino, Silvano Riva, Giulia C B Astaldi Ricotti

IRCCS Fondazione Policlinico San Matteo

Research output: Contribution to journal › Article › peer-review

Abstract

A comparison of the effects of different factors on the sensitivity of Western blotting technique to detect monoclonal antibodies is described. The major improvements were obtained by: A) renaturating the antigen in the gel before transferring it in carbonate buffer at pH 10 onto nitrocellulose and B) using alkaline-phosphatase-conjugated second antibody instead of peroxidase-conjugated second antibody.

Original language	English
Pages (from-to)	1509-1514
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	146
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(87)90820-5
Publication status	Published - Aug 14 1987

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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title = "Improvements of Western blotting to detect monoclonal antibodies",

abstract = "A comparison of the effects of different factors on the sensitivity of Western blotting technique to detect monoclonal antibodies is described. The major improvements were obtained by: A) renaturating the antigen in the gel before transferring it in carbonate buffer at pH 10 onto nitrocellulose and B) using alkaline-phosphatase-conjugated second antibody instead of peroxidase-conjugated second antibody.",

author = "Marco Bestagno and Antonella Cerino and Silvano Riva and {Astaldi Ricotti}, {Giulia C B}",

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