Improvements of Western blotting to detect monoclonal antibodies

Marco Bestagno; Antonella Cerino; Silvano Riva; Giulia C B Astaldi Ricotti

doi:10.1016/0006-291X(87)90820-5

Improvements of Western blotting to detect monoclonal antibodies

Marco Bestagno, Antonella Cerino, Silvano Riva, Giulia C B Astaldi Ricotti

IRCCS Fondazione Policlinico San Matteo

Research output: Contribution to journal › Article

Abstract

A comparison of the effects of different factors on the sensitivity of Western blotting technique to detect monoclonal antibodies is described. The major improvements were obtained by: A) renaturating the antigen in the gel before transferring it in carbonate buffer at pH 10 onto nitrocellulose and B) using alkaline-phosphatase-conjugated second antibody instead of peroxidase-conjugated second antibody.

Original language	English
Pages (from-to)	1509-1514
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	146
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(87)90820-5
Publication status	Published - Aug 14 1987

Fingerprint

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

Cite this

Bestagno, M., Cerino, A., Riva, S., & Astaldi Ricotti, G. C. B. (1987). Improvements of Western blotting to detect monoclonal antibodies. Biochemical and Biophysical Research Communications, 146(3), 1509-1514. https://doi.org/10.1016/0006-291X(87)90820-5

@article{a348e63cb5954436a95eabec316d319e,

title = "Improvements of Western blotting to detect monoclonal antibodies",

abstract = "A comparison of the effects of different factors on the sensitivity of Western blotting technique to detect monoclonal antibodies is described. The major improvements were obtained by: A) renaturating the antigen in the gel before transferring it in carbonate buffer at pH 10 onto nitrocellulose and B) using alkaline-phosphatase-conjugated second antibody instead of peroxidase-conjugated second antibody.",

author = "Marco Bestagno and Antonella Cerino and Silvano Riva and {Astaldi Ricotti}, {Giulia C B}",

year = "1987",

month = aug

day = "14",

doi = "10.1016/0006-291X(87)90820-5",

language = "English",

volume = "146",

pages = "1509--1514",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

number = "3",

}

TY - JOUR

T1 - Improvements of Western blotting to detect monoclonal antibodies

AU - Bestagno, Marco

AU - Cerino, Antonella

AU - Riva, Silvano

AU - Astaldi Ricotti, Giulia C B

PY - 1987/8/14

Y1 - 1987/8/14

N2 - A comparison of the effects of different factors on the sensitivity of Western blotting technique to detect monoclonal antibodies is described. The major improvements were obtained by: A) renaturating the antigen in the gel before transferring it in carbonate buffer at pH 10 onto nitrocellulose and B) using alkaline-phosphatase-conjugated second antibody instead of peroxidase-conjugated second antibody.

AB - A comparison of the effects of different factors on the sensitivity of Western blotting technique to detect monoclonal antibodies is described. The major improvements were obtained by: A) renaturating the antigen in the gel before transferring it in carbonate buffer at pH 10 onto nitrocellulose and B) using alkaline-phosphatase-conjugated second antibody instead of peroxidase-conjugated second antibody.

UR - http://www.scopus.com/inward/record.url?scp=0023653274&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023653274&partnerID=8YFLogxK

U2 - 10.1016/0006-291X(87)90820-5

DO - 10.1016/0006-291X(87)90820-5

M3 - Article

C2 - 3304295

AN - SCOPUS:0023653274

VL - 146

SP - 1509

EP - 1514

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -

Access to Document

10.1016/0006-291X(87)90820-5