Improvements of Western blotting to detect monoclonal antibodies

Marco Bestagno, Antonella Cerino, Silvano Riva, Giulia C B Astaldi Ricotti

Research output: Contribution to journalArticlepeer-review

Abstract

A comparison of the effects of different factors on the sensitivity of Western blotting technique to detect monoclonal antibodies is described. The major improvements were obtained by: A) renaturating the antigen in the gel before transferring it in carbonate buffer at pH 10 onto nitrocellulose and B) using alkaline-phosphatase-conjugated second antibody instead of peroxidase-conjugated second antibody.

Original languageEnglish
Pages (from-to)1509-1514
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume146
Issue number3
DOIs
Publication statusPublished - Aug 14 1987

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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