Impulse flow dependency of galanin release in vivo in the rat ventral hippocampus

Silvana Consolo, Gianni Baldi, Giovanni Russi, Gianluca Civenni, Tamas Bartfai, Annamaria Vezzani

Research output: Contribution to journalArticlepeer-review

Abstract

Using microdialysis and a sensitive RIA, we have studied the in vivo release of the neuropeptide galanin (GAL) from the ventral hippocampus of freely moving rats. The spontaneous outflow of GAL-like immunoreactivity (GAL-LI) (1.8 ± 0.3 fmol per ml per 20 min) was dependent on the presence of extracellular Ca2+ and was inhibited by tetrodotoxin. Evoked release induced by infusion of KCl (60 mM) or veratridine (148 μM) was also Ca2+- dependent and sensitive to tetrodotoxin. Electrical stimulation of the ventral limb of the diagonal band nuclei induced a frequency-dependent (50- 200 Hz) and tetrodotoxin-sensitive overflow of GAL-LI in the hippocampus. In vitro GAL-LI release (1.0 ± 0.02 fmol per ml per 5 min), studied in slices of rat ventral hippocampus, was also Ca2+-dependent and was increased in a concentration-dependent manner by KCl depolarization. This study demonstrates the release of the neuropeptide GAL in the rat central nervous system. The in vivo release is related to the activity of the cholinergic GAL-LI-containing cells in the septal diagonal band nuclei. The results are discussed in relation to the coexistence of GAL and acetylcholine within the septal/diagonal band complex.

Original languageEnglish
Pages (from-to)8047-8051
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume91
Issue number17
DOIs
Publication statusPublished - Aug 16 1994

Keywords

  • acetylcholine
  • electrical stimulation
  • galanin release in vitro
  • inhibitory neuropeptide
  • microdialysis

ASJC Scopus subject areas

  • Genetics
  • General

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