In bone metastasis miR-34a-5p absence inversely correlates with Met expression, while Met oncogene is unaffected by miR-34a-5p in non-metastatic and metastatic breast carcinomas.

Paola Maria Maroni, Rossella Puglisi, Gianfranco Mattia, Alessandra Care, Emanuela Matteucci, Paola Bendinelli, Maria Alfonsina Desiderio

Research output: Contribution to journalArticle

Abstract

The highlight of the molecular basis and therapeutic targets of the
bone-metastatic process requires the identification of biomarkers of metastasis
colonization. Here, we studied miR-34a-5p expression, and Met-receptor expression
and localization in bone metastases from ductal breast carcinomas, and in ductal
carcinomas without history of metastasis (20 cases). miR-34a-5p was elevated in
non-metastatic breast carcinoma, intermediate in the adjacent tissue and
practically absent in bone metastases, opposite to pair-matched carcinoma.
Met-receptor biomarker was highly expressed and inversely correlated with
miR-34a-5p using the same set of bone-metastasis tissues. The miR-34a-5p
silencing might depend on aberrant-epigenetic mechanisms of plastic-bone
metastases, since in 1833 cells under methyltransferase blockade miR-34a-5p
augmented. In fact, 1833 cells showed very low endogenous miR-34a-5p, in respect
to parental MDA-MB231 breast carcinoma cells, and the restoration of miR-34a-5p
with the mimic reduced Met and invasiveness. Notably, hepatocyte growth factor
(HGF)-dependent Met stabilization was observed in bone-metastatic 1833 cells,
consistent with Met co-distribution with the ligand HGF at plasma membrane and at
nuclear levels in bone metastases. Met-protein level was higher in non-metastatic
(low grade) than in metastatic (high grade) breast carcinomas, notwithstanding
miR-34a-5p-elevated expression in both the specimens. Thus, mostly in
non-metastatic carcinomas the elevated miR-34a-5p unaffected Met, important for
invasive/mesenchymal phenotype, while possibly targeting some stemness biomarkers
related to metastatic phenotype. In personalized therapies against bone
metastasis, we suggest miR-34a-5p as a suitable target of epigenetic
reprogramming leading to the accumulation of miR-34a-5p and the down-regulation
of Met-tyrosine kinase, a key player of the bone-metastatic process.
Original languageEnglish
Pages (from-to)492-503
Number of pages11
JournalCarcinogenesis
Volume38
Publication statusPublished - May 2017

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Keywords

  • Bone metastasis
  • Breast carcinomas

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