In-depth analysis of compartmentalization of HIV-1 matrix protein p17 in PBMC and plasma

Marina Selleri, Riccardo Dolcetti, Francesca Caccuri, Emanuela Giombini, Gabriella Rozera, Isabella Abbate, Alessia Mammone, Stefania Zanussi, Debora Martorelli, Simona Fiorentini, Arnaldo Caruso, Maria Rosaria Capobianchi

Research output: Contribution to journalArticle

Abstract

HIV-1 p17 plays an important role in the virus life-cycle and disease pathogenesis. Recent studies indicated a high heterogeneity of p17. A high number of insertions in the p17 carboxy-terminal region have been more frequently detected in patients with non-Hodgkin lymphoma (NHL), suggesting a role of altered p17 in lymphomagenesis. Based on p17 heterogeneity, possible PBMC/plasma compartmentalization of p17 variants was explored by ultra-deep pyrosequencing in five NHL patients. The high variability of p17 with insertions at the carboxy-terminal region was confirmed in plasma and observed for the first time in proviral genomes. Quasispecies compartmentalization was evident in 4/5 patients. Further studies are needed to define the possible role of p17 quasispecies compartmentalization in lymphomagenesis.

Original languageEnglish
Pages (from-to)58-61
Number of pages4
JournalNew Microbiologica
Volume40
Issue number1
Publication statusPublished - Jan 1 2017

Keywords

  • Compartmentalization
  • HIV-1 matrix protein
  • Non-Hodgkin lymphoma
  • P17
  • Ultra-deep pyrosequencing
  • Viral quasispecies

ASJC Scopus subject areas

  • Microbiology (medical)

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