In Ewing's sarcoma CCN3(NOV) inhibits proliferation while promoting migration and invasion of the same cell type

Stefania Benini, Bernard Perbal, Diana Zambelli, Mario Paolo Colombo, Maria Cristina Manara, Massimo Serra, Mariella Parenza, Vincent Martinez, Piero Picci, Katia Scotlandi

Research output: Contribution to journalArticlepeer-review


Altered expression of CCN3 has been observed in a variety of musculoskeletal tumours, including Ewing's sarcoma (ES). Despite its widespread distribution, very little is known about its biological functions and molecular mechanisms of action. We transfected CCN3 gene into a CCN3-negative ES cell line and analysed the in vitro and in vivo behaviours of stably transfected clones. Forced expression of CCN3 significantly reduced cell proliferation in vitro, growth in anchorage-independent conditions, and tumorigenicity in nude mice. Despite the antiproliferative effect, CCN3-transfected ES cells displayed increased migration and invasion of Matrigel. The decreased expression of α2β1 integrin receptor and the increased amount of cell surface-associated matrix metalloproteinase (MMP)-9 following the expression of CCN3 may be the basis for the increased migratory abilities of transfected cells. Cells lacking α2β1 are less facilitated to have stable anchorage since the predominant collagen extracted from ES tissue is indeed type I collagen, and proMMP-9 was recently found to provide a cellular switch between stationary and migratory ES cell phase. Our findings are in line with those recently obtained in glioblastoma. However, the underlying molecular mechanisms appear to be different, further highlighting the importance of the cellular context in the regulation of function of CCN proteins.

Original languageEnglish
Pages (from-to)4349-4361
Number of pages13
Issue number27
Publication statusPublished - Jun 23 2005


  • CCN3
  • Ewing's sarcoma
  • Migration
  • Tumorigenicity

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology


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