In house colorimetric reverse hybridisation assay for detection of the mutation most frequently associated with resistance to isoniazid in Mycobacterium tuberculosis

Mirela Verza; Raquel de Abreu Maschmann; Márcia Susana Nunes Silva; Elis Regina Dalla Costa; Marta Osório Ribeiro; Franciele Rosso; Philip Noel Suffys; Enrico Tortoli; Fiorella Marcelli; Arnaldo Zaha; Maria Lucia Rosa Rossetti

In house colorimetric reverse hybridisation assay for detection of the mutation most frequently associated with resistance to isoniazid in Mycobacterium tuberculosis

Mirela Verza, Raquel de Abreu Maschmann, Márcia Susana Nunes Silva, Elis Regina Dalla Costa, Marta Osório Ribeiro, Franciele Rosso, Philip Noel Suffys, Enrico Tortoli, Fiorella Marcelli, Arnaldo Zaha, Maria Lucia Rosa Rossetti

IRCCS Ospedale San Raffaele

Research output: Contribution to journal › Article › peer-review

Abstract

Mutations in the katG gene have been identified and correlated with isoniazid (INH) resistance in Mycobacterium tuberculosis isolates. The mutation AGC→ ACC (Ser→ Thr) at katG315 has been reported to be the most frequent and is associated with transmission and multidrug resistance. Rapid detection of this mutation could therefore improve the choice of an adequate anti-tuberculosis regimen, the epidemiological monitoring of INH resistance and, possibly, the tracking of transmission of resistant strains. An in house reverse hybridisation assay was designed in our laboratory and evaluated with 180 isolates of M. tuberculosis. It could successfully characterise the katG315 mutation in 100% of the samples as compared to DNA sequencing. The test is efficient and is a promising alternative for the rapid identification of INH resistance in regions with a high prevalence of katG315 mutants.

Original language	English
Pages (from-to)	710-714
Number of pages	5
Journal	Memorias do Instituto Oswaldo Cruz
Volume	104
Issue number	5
Publication status	Published - Aug 2009

Keywords

Drug resistance
Isoniazid
KatG315
Reverse hybridization assay
Tuberculosis

ASJC Scopus subject areas

Microbiology (medical)

Cite this

Verza, M., Maschmann, R. D. A., Silva, M. S. N., Costa, E. R. D., Ribeiro, M. O., Rosso, F., Suffys, P. N., Tortoli, E., Marcelli, F., Zaha, A., & Rossetti, M. L. R. (2009). In house colorimetric reverse hybridisation assay for detection of the mutation most frequently associated with resistance to isoniazid in Mycobacterium tuberculosis. Memorias do Instituto Oswaldo Cruz, 104(5), 710-714.

In house colorimetric reverse hybridisation assay for detection of the mutation most frequently associated with resistance to isoniazid in Mycobacterium tuberculosis. / Verza, Mirela; Maschmann, Raquel de Abreu; Silva, Márcia Susana Nunes et al.

In: Memorias do Instituto Oswaldo Cruz, Vol. 104, No. 5, 08.2009, p. 710-714.

Research output: Contribution to journal › Article › peer-review

Verza, M, Maschmann, RDA, Silva, MSN, Costa, ERD, Ribeiro, MO, Rosso, F, Suffys, PN, Tortoli, E, Marcelli, F, Zaha, A & Rossetti, MLR 2009, 'In house colorimetric reverse hybridisation assay for detection of the mutation most frequently associated with resistance to isoniazid in Mycobacterium tuberculosis', Memorias do Instituto Oswaldo Cruz, vol. 104, no. 5, pp. 710-714.

@article{5efbade03b2248469009cc0924bb91ac,

title = "In house colorimetric reverse hybridisation assay for detection of the mutation most frequently associated with resistance to isoniazid in Mycobacterium tuberculosis",

abstract = "Mutations in the katG gene have been identified and correlated with isoniazid (INH) resistance in Mycobacterium tuberculosis isolates. The mutation AGC→ ACC (Ser→ Thr) at katG315 has been reported to be the most frequent and is associated with transmission and multidrug resistance. Rapid detection of this mutation could therefore improve the choice of an adequate anti-tuberculosis regimen, the epidemiological monitoring of INH resistance and, possibly, the tracking of transmission of resistant strains. An in house reverse hybridisation assay was designed in our laboratory and evaluated with 180 isolates of M. tuberculosis. It could successfully characterise the katG315 mutation in 100% of the samples as compared to DNA sequencing. The test is efficient and is a promising alternative for the rapid identification of INH resistance in regions with a high prevalence of katG315 mutants.",

keywords = "Drug resistance, Isoniazid, KatG315, Reverse hybridization assay, Tuberculosis",

author = "Mirela Verza and Maschmann, {Raquel de Abreu} and Silva, {M{\'a}rcia Susana Nunes} and Costa, {Elis Regina Dalla} and Ribeiro, {Marta Os{\'o}rio} and Franciele Rosso and Suffys, {Philip Noel} and Enrico Tortoli and Fiorella Marcelli and Arnaldo Zaha and Rossetti, {Maria Lucia Rosa}",

year = "2009",

month = aug,

language = "English",

volume = "104",

pages = "710--714",

journal = "Memorias do Instituto Oswaldo Cruz",

issn = "0074-0276",

publisher = "Fundacao Oswaldo Cruz",

number = "5",

}

TY - JOUR

T1 - In house colorimetric reverse hybridisation assay for detection of the mutation most frequently associated with resistance to isoniazid in Mycobacterium tuberculosis

AU - Verza, Mirela

AU - Maschmann, Raquel de Abreu

AU - Silva, Márcia Susana Nunes

AU - Costa, Elis Regina Dalla

AU - Ribeiro, Marta Osório

AU - Rosso, Franciele

AU - Suffys, Philip Noel

AU - Tortoli, Enrico

AU - Marcelli, Fiorella

AU - Zaha, Arnaldo

AU - Rossetti, Maria Lucia Rosa

PY - 2009/8

Y1 - 2009/8

N2 - Mutations in the katG gene have been identified and correlated with isoniazid (INH) resistance in Mycobacterium tuberculosis isolates. The mutation AGC→ ACC (Ser→ Thr) at katG315 has been reported to be the most frequent and is associated with transmission and multidrug resistance. Rapid detection of this mutation could therefore improve the choice of an adequate anti-tuberculosis regimen, the epidemiological monitoring of INH resistance and, possibly, the tracking of transmission of resistant strains. An in house reverse hybridisation assay was designed in our laboratory and evaluated with 180 isolates of M. tuberculosis. It could successfully characterise the katG315 mutation in 100% of the samples as compared to DNA sequencing. The test is efficient and is a promising alternative for the rapid identification of INH resistance in regions with a high prevalence of katG315 mutants.

AB - Mutations in the katG gene have been identified and correlated with isoniazid (INH) resistance in Mycobacterium tuberculosis isolates. The mutation AGC→ ACC (Ser→ Thr) at katG315 has been reported to be the most frequent and is associated with transmission and multidrug resistance. Rapid detection of this mutation could therefore improve the choice of an adequate anti-tuberculosis regimen, the epidemiological monitoring of INH resistance and, possibly, the tracking of transmission of resistant strains. An in house reverse hybridisation assay was designed in our laboratory and evaluated with 180 isolates of M. tuberculosis. It could successfully characterise the katG315 mutation in 100% of the samples as compared to DNA sequencing. The test is efficient and is a promising alternative for the rapid identification of INH resistance in regions with a high prevalence of katG315 mutants.

KW - Drug resistance

KW - Isoniazid

KW - KatG315

KW - Reverse hybridization assay

KW - Tuberculosis

UR - http://www.scopus.com/inward/record.url?scp=70349696255&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70349696255&partnerID=8YFLogxK

M3 - Article

C2 - 19820830

AN - SCOPUS:70349696255

VL - 104

SP - 710

EP - 714

JO - Memorias do Instituto Oswaldo Cruz

JF - Memorias do Instituto Oswaldo Cruz

SN - 0074-0276

IS - 5

ER -

In house colorimetric reverse hybridisation assay for detection of the mutation most frequently associated with resistance to isoniazid in Mycobacterium tuberculosis

Abstract

Keywords

ASJC Scopus subject areas

Other files and links

Fingerprint

Cite this