TY - JOUR
T1 - In house colorimetric reverse hybridisation assay for detection of the mutation most frequently associated with resistance to isoniazid in Mycobacterium tuberculosis
AU - Verza, Mirela
AU - Maschmann, Raquel de Abreu
AU - Silva, Márcia Susana Nunes
AU - Costa, Elis Regina Dalla
AU - Ribeiro, Marta Osório
AU - Rosso, Franciele
AU - Suffys, Philip Noel
AU - Tortoli, Enrico
AU - Marcelli, Fiorella
AU - Zaha, Arnaldo
AU - Rossetti, Maria Lucia Rosa
PY - 2009/8
Y1 - 2009/8
N2 - Mutations in the katG gene have been identified and correlated with isoniazid (INH) resistance in Mycobacterium tuberculosis isolates. The mutation AGC→ ACC (Ser→ Thr) at katG315 has been reported to be the most frequent and is associated with transmission and multidrug resistance. Rapid detection of this mutation could therefore improve the choice of an adequate anti-tuberculosis regimen, the epidemiological monitoring of INH resistance and, possibly, the tracking of transmission of resistant strains. An in house reverse hybridisation assay was designed in our laboratory and evaluated with 180 isolates of M. tuberculosis. It could successfully characterise the katG315 mutation in 100% of the samples as compared to DNA sequencing. The test is efficient and is a promising alternative for the rapid identification of INH resistance in regions with a high prevalence of katG315 mutants.
AB - Mutations in the katG gene have been identified and correlated with isoniazid (INH) resistance in Mycobacterium tuberculosis isolates. The mutation AGC→ ACC (Ser→ Thr) at katG315 has been reported to be the most frequent and is associated with transmission and multidrug resistance. Rapid detection of this mutation could therefore improve the choice of an adequate anti-tuberculosis regimen, the epidemiological monitoring of INH resistance and, possibly, the tracking of transmission of resistant strains. An in house reverse hybridisation assay was designed in our laboratory and evaluated with 180 isolates of M. tuberculosis. It could successfully characterise the katG315 mutation in 100% of the samples as compared to DNA sequencing. The test is efficient and is a promising alternative for the rapid identification of INH resistance in regions with a high prevalence of katG315 mutants.
KW - Drug resistance
KW - Isoniazid
KW - KatG315
KW - Reverse hybridization assay
KW - Tuberculosis
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M3 - Article
C2 - 19820830
AN - SCOPUS:70349696255
VL - 104
SP - 710
EP - 714
JO - Memorias do Instituto Oswaldo Cruz
JF - Memorias do Instituto Oswaldo Cruz
SN - 0074-0276
IS - 5
ER -