In leukaemic CD5+ B cells the expression of BCL-2 gene family is shifted toward protection from apoptosis

D. Gottardi, A. Alfarano, A. M. De Leo, A. Stacchini, M. Aragno, A. Rigo, D. Veneri, R. Zanotti, G. Pizzolo, F. Caligaris-Cappio

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Abstract

This study further investigated the mechanisms that control apoptosis in leukaemic CD5+ B cells, and focused on the Bcl-2 gene family. The pattern of expression of Bcl-2, Bcl-xL, Bcl-xS and Bax genes, selected because of their interrelated role in the control of apoptosis, was analysed in a series of CD5+ B-cell chronic lymphoid leukaemias. Cells from 34 patients with chronic lymphoid leukaemia of B-cell type (23 B-chronic lymphocytic leukaemia (B-CLL) and 11 mantle cell lymphoma (MCL) in leukaemic phase) were investigated. High levels of Bcl-2 mRNA were observed by Northern blot and high levels of Bcl-2 protein were detected by cytofluorograph analysis with a specific monoclonal antibody (MAb) in all cases. Strong Bax expression was detected by RT-PCR in 20/23 B-CLL cases; Bax was also observed in 8/11 MCL in leukaemic phase with variable degree of intensity. In both B-CLL and MCL samples the presence of Bar protein was confirmed by cytofluorograph analysis. RT-PCR detected high levels of Bcl-xL in 16/23 B-CLL and in 8/11 MCL in leukaemic phase, whereas Bcl-xS was detectable in low to trace amounts respectively in 13/23 B-CLL and in 6/11 MCL in leukaemic phase. According to the functional role of Bcl-2, Bcl-xL, Bcl-xS and Bax, these data indicate that the pattern of Bcl-2 family genes expression in leukaemic CD5+ B cells is skewed toward prevention of apoptosis and may thus favour the relentless accumulation of CD5+ leukaemic B cells.

Original languageEnglish
Pages (from-to)612-618
Number of pages7
JournalBritish Journal of Haematology
Volume94
Issue number4
Publication statusPublished - 1996

Fingerprint

Mantle-Cell Lymphoma
B-Cell Chronic Lymphocytic Leukemia
B-Lymphocytes
Apoptosis
Lymphoid Leukemia
bcl-2 Genes
Genes
Polymerase Chain Reaction
Northern Blotting
Proteins
Monoclonal Antibodies
Gene Expression
Messenger RNA

Keywords

  • Apoptosis
  • B-chronic lymphocytic leukaemia
  • Bcl-2 gene family
  • CD5 B cells
  • Mantle cell lymphoma

ASJC Scopus subject areas

  • Hematology

Cite this

Gottardi, D., Alfarano, A., De Leo, A. M., Stacchini, A., Aragno, M., Rigo, A., ... Caligaris-Cappio, F. (1996). In leukaemic CD5+ B cells the expression of BCL-2 gene family is shifted toward protection from apoptosis. British Journal of Haematology, 94(4), 612-618.

In leukaemic CD5+ B cells the expression of BCL-2 gene family is shifted toward protection from apoptosis. / Gottardi, D.; Alfarano, A.; De Leo, A. M.; Stacchini, A.; Aragno, M.; Rigo, A.; Veneri, D.; Zanotti, R.; Pizzolo, G.; Caligaris-Cappio, F.

In: British Journal of Haematology, Vol. 94, No. 4, 1996, p. 612-618.

Research output: Contribution to journalArticle

Gottardi, D, Alfarano, A, De Leo, AM, Stacchini, A, Aragno, M, Rigo, A, Veneri, D, Zanotti, R, Pizzolo, G & Caligaris-Cappio, F 1996, 'In leukaemic CD5+ B cells the expression of BCL-2 gene family is shifted toward protection from apoptosis', British Journal of Haematology, vol. 94, no. 4, pp. 612-618.
Gottardi D, Alfarano A, De Leo AM, Stacchini A, Aragno M, Rigo A et al. In leukaemic CD5+ B cells the expression of BCL-2 gene family is shifted toward protection from apoptosis. British Journal of Haematology. 1996;94(4):612-618.
Gottardi, D. ; Alfarano, A. ; De Leo, A. M. ; Stacchini, A. ; Aragno, M. ; Rigo, A. ; Veneri, D. ; Zanotti, R. ; Pizzolo, G. ; Caligaris-Cappio, F. / In leukaemic CD5+ B cells the expression of BCL-2 gene family is shifted toward protection from apoptosis. In: British Journal of Haematology. 1996 ; Vol. 94, No. 4. pp. 612-618.
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abstract = "This study further investigated the mechanisms that control apoptosis in leukaemic CD5+ B cells, and focused on the Bcl-2 gene family. The pattern of expression of Bcl-2, Bcl-xL, Bcl-xS and Bax genes, selected because of their interrelated role in the control of apoptosis, was analysed in a series of CD5+ B-cell chronic lymphoid leukaemias. Cells from 34 patients with chronic lymphoid leukaemia of B-cell type (23 B-chronic lymphocytic leukaemia (B-CLL) and 11 mantle cell lymphoma (MCL) in leukaemic phase) were investigated. High levels of Bcl-2 mRNA were observed by Northern blot and high levels of Bcl-2 protein were detected by cytofluorograph analysis with a specific monoclonal antibody (MAb) in all cases. Strong Bax expression was detected by RT-PCR in 20/23 B-CLL cases; Bax was also observed in 8/11 MCL in leukaemic phase with variable degree of intensity. In both B-CLL and MCL samples the presence of Bar protein was confirmed by cytofluorograph analysis. RT-PCR detected high levels of Bcl-xL in 16/23 B-CLL and in 8/11 MCL in leukaemic phase, whereas Bcl-xS was detectable in low to trace amounts respectively in 13/23 B-CLL and in 6/11 MCL in leukaemic phase. According to the functional role of Bcl-2, Bcl-xL, Bcl-xS and Bax, these data indicate that the pattern of Bcl-2 family genes expression in leukaemic CD5+ B cells is skewed toward prevention of apoptosis and may thus favour the relentless accumulation of CD5+ leukaemic B cells.",
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