In ovo injection of a galacto-oligosaccharide prebiotic in broiler chickens submitted to heat-stress: Impact on transcriptomic profile and plasma immune parameters

Micol Bertocchi, Marco Zampiga, Diana Luise, Marika Vitali, Federico Sirri, Anna Slawinska, Siria Tavaniello, Orazio Palumbo, Ivonne Archetti, Giuseppe Maiorano, Paolo Bosi, Paolo Trevisi

Research output: Contribution to journalArticlepeer-review

Abstract

This study investigated the effects of a galactooligosaccharide (GOS) prebiotic in ovo injected on intestinal transcriptome and plasma immune parameters of broiler chickens kept under thermoneutral (TN) or heat stress (HS) conditions. Fertilized Ross 308 eggs were injected in ovo with 0.2 mL physiological saline without (control, CON) or with 3.5 mg of GOS (GOS). Three-hundred male chicks/injection treatment (25 birds/pen) were kept in TN or HS (30 °C) conditions during the last growing phase, in a 2 × 2 factorial design. At slaughter, from 20 birds/injection group (half from TN and half from HS), jejunum and cecum were collected for transcriptome analysis, and plasma was collected. No differences in plasma parameters (IgA and IgG, serum amyloid) and no interaction between injection treatment and environment condition were found. GOS-enriched gene sets related to energetic metabolism in jejunum, and to lipid metabolism in cecum, were involved in gut barrier maintenance. A homogeneous reaction to heat stress was determined along the gut,.

Original languageEnglish
Article number1067
JournalAnimals
Volume9
Issue number12
DOIs
Publication statusPublished - Dec 2019

Keywords

  • Galactooligosaccharides
  • Heat stress
  • In ovo injection
  • Intestine
  • Transcriptome

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

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