TY - JOUR
T1 - In patients with myelodysplastic syndromes response to rHuEPO and G-CSF treatment is related to an increase of cytogenetically normal CD34+ cells
AU - Rigolin, Gian Matteo
AU - Porta, Matteo Della
AU - Ciccone, Maria
AU - Bugli, Anna Maria
AU - Bragotti, Letizia Zenone
AU - Mauro, Endri
AU - Fraulini, Chiara
AU - Rossi, Antonella Russo
AU - Bardi, Antonella
AU - Cuneo, Antonio
AU - Castoldi, Gianluigi
PY - 2004/8
Y1 - 2004/8
N2 - The in vivo effect of recombinant human erythropoietin (rHuEpo) and granulocyte colony-stimulating factor (G-CSF) combined treatment on CD34 + cells was evaluated by fluorescence in situ hybridization (FISH) in 13 myelodysplastic syndrome (MDS) patients with known cytogenetic abnormalities. After treatment, responsive patients presented a significantly lower proportion of FISH abnormal CD34+ cells than before treatment (P = 0.003), and in comparison with unresponsive cases (P = 0.007). Response to treatment was associated with a reduced degree of apoptosis in CD34+ cells (P = 0.021): however, no difference in telomere length was observed in responsive patients after growth factor administration. Although the number of patients analysed was relatively small, the present data suggest that, in MDS patients, response to rHuEpo and G-CSF may be related to the proliferation of karyotypically normal but potentially defective CD34+ progenitor cells.
AB - The in vivo effect of recombinant human erythropoietin (rHuEpo) and granulocyte colony-stimulating factor (G-CSF) combined treatment on CD34 + cells was evaluated by fluorescence in situ hybridization (FISH) in 13 myelodysplastic syndrome (MDS) patients with known cytogenetic abnormalities. After treatment, responsive patients presented a significantly lower proportion of FISH abnormal CD34+ cells than before treatment (P = 0.003), and in comparison with unresponsive cases (P = 0.007). Response to treatment was associated with a reduced degree of apoptosis in CD34+ cells (P = 0.021): however, no difference in telomere length was observed in responsive patients after growth factor administration. Although the number of patients analysed was relatively small, the present data suggest that, in MDS patients, response to rHuEpo and G-CSF may be related to the proliferation of karyotypically normal but potentially defective CD34+ progenitor cells.
KW - CD34 cells
KW - Fluorescence in situ hybridization
KW - Granulocyte colony-stimulating factor
KW - Myelodysplastic syndromes
KW - Recombinant human erythropoietin
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U2 - 10.1111/j.1365-2141.2004.05086.x
DO - 10.1111/j.1365-2141.2004.05086.x
M3 - Article
C2 - 15287942
AN - SCOPUS:4143121032
VL - 126
SP - 501
EP - 507
JO - British Journal of Haematology
JF - British Journal of Haematology
SN - 0007-1048
IS - 4
ER -