In patients with myelodysplastic syndromes response to rHuEPO and G-CSF treatment is related to an increase of cytogenetically normal CD34+ cells

Gian Matteo Rigolin, Matteo Della Porta, Maria Ciccone, Anna Maria Bugli, Letizia Zenone Bragotti, Endri Mauro, Chiara Fraulini, Antonella Russo Rossi, Antonella Bardi, Antonio Cuneo, Gianluigi Castoldi

Research output: Contribution to journalArticlepeer-review

Abstract

The in vivo effect of recombinant human erythropoietin (rHuEpo) and granulocyte colony-stimulating factor (G-CSF) combined treatment on CD34 + cells was evaluated by fluorescence in situ hybridization (FISH) in 13 myelodysplastic syndrome (MDS) patients with known cytogenetic abnormalities. After treatment, responsive patients presented a significantly lower proportion of FISH abnormal CD34+ cells than before treatment (P = 0.003), and in comparison with unresponsive cases (P = 0.007). Response to treatment was associated with a reduced degree of apoptosis in CD34+ cells (P = 0.021): however, no difference in telomere length was observed in responsive patients after growth factor administration. Although the number of patients analysed was relatively small, the present data suggest that, in MDS patients, response to rHuEpo and G-CSF may be related to the proliferation of karyotypically normal but potentially defective CD34+ progenitor cells.

Original languageEnglish
Pages (from-to)501-507
Number of pages7
JournalBritish Journal of Haematology
Volume126
Issue number4
DOIs
Publication statusPublished - Aug 2004

Keywords

  • CD34 cells
  • Fluorescence in situ hybridization
  • Granulocyte colony-stimulating factor
  • Myelodysplastic syndromes
  • Recombinant human erythropoietin

ASJC Scopus subject areas

  • Hematology

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