In silico investigation of the molecular effects caused by R123H variant in secretory phospholipase A2-IIA associated with ARDS

Benedetta Righino, Angelo Minucci, Davide Pirolli, Ettore Capoluongo, Giorgio Conti, Daniele De Luca, Maria Cristina De Rosa

Research output: Contribution to journalArticle

Abstract

Phospholipase A2-IIA catalyzes the hydrolysis of the sn-2 ester of glycerophospholipids. A rare c.428G > A (p.Arg143His) variant in PLA2G2A gene was found in two infants affected by acute respiratory distress syndrome (ARDS) by whole coding region and exon/intron boundaries sequencing. To obtain insights into the possible molecular effects of the rare R123H mutation in secretory PLA2-IIA (sPLA2-IIA), molecular modelling, molecular dynamics (MD) using principal component analysis (PCA) and continuum electrostatic calculations were conducted on the crystal structure of the wild type protein and on a generated model structure of the R123H mutant. Analysis of MD trajectories indicate that the overall stability of the protein is not affected by this mutation but nevertheless the catalytically crucial H-bond between Tyr51 and Asp91 as well as main electrostatic interactions in the region close to the mutation site are altered. PCA results indicate that the R123H replacement alter the internal molecular motions of the enzyme and that collective motions are increased. Electrostatic surface potential studies suggest that after mutation the interfacial binding to anionic phospholipid membranes and anionic proteins may be changed. The strengthening of electrostatic interactions may be propagated into the active site region thus potentially affecting the substrate recognition and enzymatic activity. Our findings provide the basis for further investigation and advances our understanding of the effects of mutations on sPLA2 structure and function.

Original languageEnglish
Pages (from-to)68-76
Number of pages9
JournalJournal of Molecular Graphics and Modelling
Volume81
DOIs
Publication statusPublished - May 1 2018

Fingerprint

Secretory Phospholipase A2
mutations
Coulomb interactions
Proteins
Principal component analysis
Molecular dynamics
Electrostatics
electrostatics
Glycerophospholipids
principal components analysis
proteins
Molecular modeling
Phospholipases A2
Phospholipids
Surface potential
Model structures
Introns
Exons
Hydrolysis
Esters

Keywords

  • Electrostatic potential
  • Molecular dynamics simulation
  • Molecular modelling
  • PLA2G2A gene
  • sPLA2

ASJC Scopus subject areas

  • Spectroscopy
  • Physical and Theoretical Chemistry
  • Computer Graphics and Computer-Aided Design
  • Materials Chemistry

Cite this

In silico investigation of the molecular effects caused by R123H variant in secretory phospholipase A2-IIA associated with ARDS. / Righino, Benedetta; Minucci, Angelo; Pirolli, Davide; Capoluongo, Ettore; Conti, Giorgio; De Luca, Daniele; De Rosa, Maria Cristina.

In: Journal of Molecular Graphics and Modelling, Vol. 81, 01.05.2018, p. 68-76.

Research output: Contribution to journalArticle

Righino, Benedetta ; Minucci, Angelo ; Pirolli, Davide ; Capoluongo, Ettore ; Conti, Giorgio ; De Luca, Daniele ; De Rosa, Maria Cristina. / In silico investigation of the molecular effects caused by R123H variant in secretory phospholipase A2-IIA associated with ARDS. In: Journal of Molecular Graphics and Modelling. 2018 ; Vol. 81. pp. 68-76.
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