TY - JOUR
T1 - In silico prediction and experimental validation of natural antisense transcripts in two cancer-associated regions of human chromosome 6
AU - Monti, Laura
AU - Cinquetti, Raffaella
AU - Guffanti, Alessandro
AU - Nicassio, Francesco
AU - Cremona, Mattia
AU - Lavorgna, Giovanni
AU - Bianchi, Fabrizio
AU - Vignati, Francesca
AU - Cittaro, Davide
AU - Taramelli, Roberto
AU - Acquati, Francesco
PY - 2009
Y1 - 2009
N2 - Antisense transcription has long been recognized as a mechanism involved in the regulation of gene expression. Therefore, several human diseases associated with abnormal patterns of gene expression might display antisense RNA-mediated pathogenetic mechanisms. Such issue could be particularly relevant for cancer pathogenesis, since deregulated gene expression has long been established as a hallmark of cancer cells. Herein, we report on a bioinformatic search for antisense transcription in two cancer-associated regions of human chromosome 6 (6q21 and 6q27). Natural antisense transcripts (NATs) for several genes in both genomic regions were predicted in silico and subsequently validated by strand-specific RT-PCR. Detailed experimental validation by quantitative real-time RT-PCR of five putative cancer related sense-antisense transcript pairs revealed a single candidate tumor suppressor gene (RPS6KA2) whose expression levels display marked cancer-related changes that are likely mediated by its antisense RNA in a breast cancer cell line model.
AB - Antisense transcription has long been recognized as a mechanism involved in the regulation of gene expression. Therefore, several human diseases associated with abnormal patterns of gene expression might display antisense RNA-mediated pathogenetic mechanisms. Such issue could be particularly relevant for cancer pathogenesis, since deregulated gene expression has long been established as a hallmark of cancer cells. Herein, we report on a bioinformatic search for antisense transcription in two cancer-associated regions of human chromosome 6 (6q21 and 6q27). Natural antisense transcripts (NATs) for several genes in both genomic regions were predicted in silico and subsequently validated by strand-specific RT-PCR. Detailed experimental validation by quantitative real-time RT-PCR of five putative cancer related sense-antisense transcript pairs revealed a single candidate tumor suppressor gene (RPS6KA2) whose expression levels display marked cancer-related changes that are likely mediated by its antisense RNA in a breast cancer cell line model.
KW - Antisense RNA
KW - Chromosome 6
KW - Transcriptional regulation
KW - Tumor suppressor genes
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U2 - 10.3892/ijo_00000237
DO - 10.3892/ijo_00000237
M3 - Article
C2 - 19287968
AN - SCOPUS:67649451972
VL - 34
SP - 1099
EP - 1108
JO - International Journal of Oncology
JF - International Journal of Oncology
SN - 1019-6439
IS - 4
ER -