In situ detection of cytomegalovirus DNA in biopsies of AIDS patients using a hybrido-immunocytochemical assay

M. Musiani, G. Gentilomi, M. Zerbini, D. Gibellini, G. Gallinella, S. Pileri, P. Baglioni, M. La Placa

Research output: Contribution to journalArticle

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Abstract

An in situ hybrido-immunocytochemical assay, with a digoxigenin-labelled probe, was used to show the presence of cytomegalovirus DNA in both paraffin and frozen sections from tissue blocks of 5 AIDS patients. The hybridization probe was constructed by using two different DNA fragments of the repeated sequences of the CMV genome. The CMV DNA probe hybridized in situ was immunocytochemically visualized by anti-digoxigenin Fab fragments labelled with alkaline phosphatase. This hybridization procedure proved to be sensitive, specific, and provided good resolving power. Thus, it might effectively be employed in immunohistological and virological laboratories for the diagnosis of CMV infections in AIDS patients; indeed it might even be applied further in the virological context.

Original languageEnglish
Pages (from-to)21-25
Number of pages5
JournalHistochemistry
Volume94
Issue number1
DOIs
Publication statusPublished - May 1990

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Digoxigenin
Cytomegalovirus
Acquired Immunodeficiency Syndrome
Biopsy
Immunoglobulin Fab Fragments
Clinical Laboratory Techniques
DNA
DNA Probes
Frozen Sections
Paraffin
Alkaline Phosphatase
Genome
Infection

ASJC Scopus subject areas

  • Anatomy

Cite this

Musiani, M., Gentilomi, G., Zerbini, M., Gibellini, D., Gallinella, G., Pileri, S., ... La Placa, M. (1990). In situ detection of cytomegalovirus DNA in biopsies of AIDS patients using a hybrido-immunocytochemical assay. Histochemistry, 94(1), 21-25. https://doi.org/10.1007/BF00266785

In situ detection of cytomegalovirus DNA in biopsies of AIDS patients using a hybrido-immunocytochemical assay. / Musiani, M.; Gentilomi, G.; Zerbini, M.; Gibellini, D.; Gallinella, G.; Pileri, S.; Baglioni, P.; La Placa, M.

In: Histochemistry, Vol. 94, No. 1, 05.1990, p. 21-25.

Research output: Contribution to journalArticle

Musiani, M, Gentilomi, G, Zerbini, M, Gibellini, D, Gallinella, G, Pileri, S, Baglioni, P & La Placa, M 1990, 'In situ detection of cytomegalovirus DNA in biopsies of AIDS patients using a hybrido-immunocytochemical assay', Histochemistry, vol. 94, no. 1, pp. 21-25. https://doi.org/10.1007/BF00266785
Musiani, M. ; Gentilomi, G. ; Zerbini, M. ; Gibellini, D. ; Gallinella, G. ; Pileri, S. ; Baglioni, P. ; La Placa, M. / In situ detection of cytomegalovirus DNA in biopsies of AIDS patients using a hybrido-immunocytochemical assay. In: Histochemistry. 1990 ; Vol. 94, No. 1. pp. 21-25.
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