In situ hybridization analysis of preprotachykinin-A mRNA levels in young and old rats

P. Pompei, R. Severini, G. Costa, M. Massi, P. Fattoretti, C. Bertoni-Freddari

Research output: Contribution to journalArticlepeer-review

Abstract

The present study examined the effects of ageing on preprotachykinin-A (PPT-A) mRNA levels in discrete regions of the rat brain. Semiquantitative analysis of silver grains revealed a 16% statistically significant decrease in PPT-A mRNA in the shell of the nucleus accumbens (AcbSh), a 27.6% statistically significant lower level of PPT-A mRNA in the olfactory tubercle (Tu), a 19.2% and 31.5% statistically significant decrease in PPT-A mRNA in the dorsal and ventral caudate-putamen (d-CPu) (v-CPu), respectively, a 30% statistically significant lower expression of PPT-A mRNA in the bed nucleus of the stria terminalis (BNST), a 33.7% statistically significant decrease in PPT-A mRNA in the habenula (Hb) and a 30% statistically significant decrease of PPT-A mRNA levels in the postero-dorsal part of the medial amygdala (MePD). No changes in PPT-A mRNA levels were found in the nucleus accumbens, core (AcbC), in the islands of Calleja (Icj), and in the medial preoptic area (mPOA). These results show that ageing of the central nervous system (CNS) is associated with widespread changes in tachykinin gene expression, suggesting that alteration in the tachykinergic system may have implications in the physio-pathology of the elderly.

Original languageEnglish
Pages (from-to)132-136
Number of pages5
JournalMolecular Brain Research
Volume64
Issue number1
DOIs
Publication statusPublished - Jan 22 1999

Keywords

  • Age
  • Brain area
  • Preprotachykinin-A mRNA
  • Young/old rat

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

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