In situ hybridization of albumin mRNA in normal liver and liver tumors: Identification of hepatocellular origin

Koji Yamaguchi, Michael A. Nalesnik, Brian I. Carr

Research output: Contribution to journalArticlepeer-review

Abstract

In situ hybridization was performed to detect albumin mRNA in normal liver, liver cirrhosis, primary liver tumors and secondary liver neoplasms. In areas of normal liver, and liver cirrhosis, signals for albumin mRNA were present in hepatocytes, whereas no signals were seen in other cells such as endothelial and Küpffer cells, bile duct epithelium and smooth muscle cells. In 53 of 56 hepatocellular carcinomas signals were present in tumor cells but in eight cholangiocarcinomas and 14 metastatic adenocarcinomas from large bowel or pancreas, carcinoma cells were negative for albumin mRNA. In three metastatic tumors (from two neuroendocrine carcinomas and one gastric leiomyosarcoma), tumor cells contained no signals, while the surrounding hepatocytes showed diffuse grains. In 15 of the 84 specimens examined in situ hybridization was applied to routine formalin-fixed and paraffin-embedded blocks and strong signals were obtained for albumin mRNA. We conclude that in situ hybridization of human albumin is a valid tool in the differential diagnosis of hepatocellular carcinoma from cholangiocarcinomas and tumors metastatic to the liver.

Original languageEnglish
Pages (from-to)361-365
Number of pages5
JournalVirchows Archiv B Cell Pathology Including Molecular Pathology
Volume64
Issue number1
DOIs
Publication statusPublished - Dec 1993

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Fingerprint Dive into the research topics of 'In situ hybridization of albumin mRNA in normal liver and liver tumors: Identification of hepatocellular origin'. Together they form a unique fingerprint.

Cite this