Abstract
Distribution of protein zero (P0) and myelin basic protein (MBP) mRNAs in the sciatic nerve from rats with alloxan-induced diabetes was analyzed at two different time points using in situ hybridization. Some animals of each diabetic group were treated with insulin. Densitometric quantitation of silver clusters revealed that 5 weeks after diabetes induction P0 mRNA only is significantly increased, while at 14 weeks both P0 and MBP mRNA contents are markedly higher than controls. Insulin treatment normalizes glycemia levels and slightly counteracts increased P0 mRNA at both stages of diabetes. An increase in MBP mRNA is observed in chronic diabetic animals only, and is unaltered by the normoglycemic effect of insulin. The increased transcript levels of P0 and MBP suggest that Schwann cells can modulate gene expression of myelin-specific proteins in response to diabetic-induced metabolic derangement. Such a change may represent a higher turnover of myelin proteins as an attempt by the Schwann cells to repair the diabetes-induced nerve damage. The observed pattern of transcript amount is only slightly influenced by insulin treatment.
| Original language | English |
|---|---|
| Pages (from-to) | 65-69 |
| Number of pages | 5 |
| Journal | Neuroscience Letters |
| Volume | 207 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Mar 22 1996 |
Fingerprint
Keywords
- Experimental diabetic neuropathy
- In situ hybridization
- Myelin basic protein (MBP)
- Myelin-induced diabetes
- Peripheral nerve
- Protein zero (P)
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
In situ hybridization study of myelin protein mRNA in rats with an experimental diabetic neuropathy. / Conti, Anna Maria; Scarpini, Elio; Malosio, Maria Luisa; Di Giulio, Anna Maria; Baron, Pierluigi; Scarlato, Guglielmo; Mantegazza, Paolo; Gorio, Alfredo.
In: Neuroscience Letters, Vol. 207, No. 1, 22.03.1996, p. 65-69.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - In situ hybridization study of myelin protein mRNA in rats with an experimental diabetic neuropathy
AU - Conti, Anna Maria
AU - Scarpini, Elio
AU - Malosio, Maria Luisa
AU - Di Giulio, Anna Maria
AU - Baron, Pierluigi
AU - Scarlato, Guglielmo
AU - Mantegazza, Paolo
AU - Gorio, Alfredo
PY - 1996/3/22
Y1 - 1996/3/22
N2 - Distribution of protein zero (P0) and myelin basic protein (MBP) mRNAs in the sciatic nerve from rats with alloxan-induced diabetes was analyzed at two different time points using in situ hybridization. Some animals of each diabetic group were treated with insulin. Densitometric quantitation of silver clusters revealed that 5 weeks after diabetes induction P0 mRNA only is significantly increased, while at 14 weeks both P0 and MBP mRNA contents are markedly higher than controls. Insulin treatment normalizes glycemia levels and slightly counteracts increased P0 mRNA at both stages of diabetes. An increase in MBP mRNA is observed in chronic diabetic animals only, and is unaltered by the normoglycemic effect of insulin. The increased transcript levels of P0 and MBP suggest that Schwann cells can modulate gene expression of myelin-specific proteins in response to diabetic-induced metabolic derangement. Such a change may represent a higher turnover of myelin proteins as an attempt by the Schwann cells to repair the diabetes-induced nerve damage. The observed pattern of transcript amount is only slightly influenced by insulin treatment.
AB - Distribution of protein zero (P0) and myelin basic protein (MBP) mRNAs in the sciatic nerve from rats with alloxan-induced diabetes was analyzed at two different time points using in situ hybridization. Some animals of each diabetic group were treated with insulin. Densitometric quantitation of silver clusters revealed that 5 weeks after diabetes induction P0 mRNA only is significantly increased, while at 14 weeks both P0 and MBP mRNA contents are markedly higher than controls. Insulin treatment normalizes glycemia levels and slightly counteracts increased P0 mRNA at both stages of diabetes. An increase in MBP mRNA is observed in chronic diabetic animals only, and is unaltered by the normoglycemic effect of insulin. The increased transcript levels of P0 and MBP suggest that Schwann cells can modulate gene expression of myelin-specific proteins in response to diabetic-induced metabolic derangement. Such a change may represent a higher turnover of myelin proteins as an attempt by the Schwann cells to repair the diabetes-induced nerve damage. The observed pattern of transcript amount is only slightly influenced by insulin treatment.
KW - Experimental diabetic neuropathy
KW - In situ hybridization
KW - Myelin basic protein (MBP)
KW - Myelin-induced diabetes
KW - Peripheral nerve
KW - Protein zero (P)
UR - http://www.scopus.com/inward/record.url?scp=0029875045&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029875045&partnerID=8YFLogxK
U2 - 10.1016/0304-3940(96)12490-3
DO - 10.1016/0304-3940(96)12490-3
M3 - Article
C2 - 8710212
AN - SCOPUS:0029875045
VL - 207
SP - 65
EP - 69
JO - Neuroscience Letters
JF - Neuroscience Letters
SN - 0304-3940
IS - 1
ER -