In situ hybridization study of myelin protein mRNA in rats with an experimental diabetic neuropathy

Anna Maria Conti, Elio Scarpini, Maria Luisa Malosio, Anna Maria Di Giulio, Pierluigi Baron, Guglielmo Scarlato, Paolo Mantegazza, Alfredo Gorio

Research output: Contribution to journalArticle

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Abstract

Distribution of protein zero (P0) and myelin basic protein (MBP) mRNAs in the sciatic nerve from rats with alloxan-induced diabetes was analyzed at two different time points using in situ hybridization. Some animals of each diabetic group were treated with insulin. Densitometric quantitation of silver clusters revealed that 5 weeks after diabetes induction P0 mRNA only is significantly increased, while at 14 weeks both P0 and MBP mRNA contents are markedly higher than controls. Insulin treatment normalizes glycemia levels and slightly counteracts increased P0 mRNA at both stages of diabetes. An increase in MBP mRNA is observed in chronic diabetic animals only, and is unaltered by the normoglycemic effect of insulin. The increased transcript levels of P0 and MBP suggest that Schwann cells can modulate gene expression of myelin-specific proteins in response to diabetic-induced metabolic derangement. Such a change may represent a higher turnover of myelin proteins as an attempt by the Schwann cells to repair the diabetes-induced nerve damage. The observed pattern of transcript amount is only slightly influenced by insulin treatment.

Original languageEnglish
Pages (from-to)65-69
Number of pages5
JournalNeuroscience Letters
Volume207
Issue number1
DOIs
Publication statusPublished - Mar 22 1996

Fingerprint

Myelin Proteins
Diabetic Neuropathies
Myelin Basic Protein
In Situ Hybridization
Messenger RNA
Insulin
Schwann Cells
Myelin P0 Protein
Experimental Diabetes Mellitus
Sciatic Nerve
Silver
Gene Expression

Keywords

  • Experimental diabetic neuropathy
  • In situ hybridization
  • Myelin basic protein (MBP)
  • Myelin-induced diabetes
  • Peripheral nerve
  • Protein zero (P)

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

In situ hybridization study of myelin protein mRNA in rats with an experimental diabetic neuropathy. / Conti, Anna Maria; Scarpini, Elio; Malosio, Maria Luisa; Di Giulio, Anna Maria; Baron, Pierluigi; Scarlato, Guglielmo; Mantegazza, Paolo; Gorio, Alfredo.

In: Neuroscience Letters, Vol. 207, No. 1, 22.03.1996, p. 65-69.

Research output: Contribution to journalArticle

Conti, Anna Maria ; Scarpini, Elio ; Malosio, Maria Luisa ; Di Giulio, Anna Maria ; Baron, Pierluigi ; Scarlato, Guglielmo ; Mantegazza, Paolo ; Gorio, Alfredo. / In situ hybridization study of myelin protein mRNA in rats with an experimental diabetic neuropathy. In: Neuroscience Letters. 1996 ; Vol. 207, No. 1. pp. 65-69.
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