In situ RT-PCR allows the detection of ornithine decarboxylase mRNA in paraffin embedded archival human hyperplastic breast tissues

L. Clerico, T. Mancuso, L. Da Prato, N. Marziliano, S. Garagna, V. Pecile, E. Demori, M. Morgutti, A. Città, S. Parodi, A. Amoroso, S. Crovella

Research output: Contribution to journalArticlepeer-review

Abstract

Expression of ornithine decarboxylase (ODC) is induced by c-Myc oncoprotein and is required for cell proliferation and tumour growth. We have studied the expression of ODC mRNA by in situ hybridisation and in situ RT-PCR in archival human hyperplastic breast tissues. A very low signal was detected by in situ hybridisation, while the in situ RT-PCR on human breast archival tissues demonstrated an over-expression of ODC mRNA in epithelial cells characterised by some degree of hyperplasia, maintaining the morphology of the archival tissue intact despite the multiple steps of fixation, permeabilization and thermal cycling.

Original languageEnglish
Pages (from-to)179-183
Number of pages5
JournalEuropean journal of histochemistry : EJH
Volume43
Issue number3
Publication statusPublished - 1999

Keywords

  • Archival tissues
  • Hyperplastic breast tissue
  • In situ PCR
  • MRNA expression
  • Ornithine decarboxilase

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Cell Biology
  • Developmental Biology
  • Anatomy

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