In vitro activity and in vivo efficacy of the saponin diosgenyl 2-amino-2-deoxy-b-d-glucopyranoside hydrochloride (HSM1) alone and in combination with daptomycin and vancomycin against Gram-positive cocci

Oscar Cirioni, Henryk Myszka, Malgorzata Dawgul, Roberto Ghiselli, Fiorenza Orlando, Carmela Silvestri, Lucia Brescini, Wojciech Kamysz, Mario Guerrieri, Andrea Giacometti

Research output: Contribution to journalArticle

Abstract

Surgical site infections are the second most common hospital-and community-acquired Grampositive infections, with the US Centers for Disease Control and Prevention estimating that about 500 000 surgical site infections occur annually in the USA. The aim of this work was to determine the in vitro activity of the saponin diosgenyl 2-amino-2-deoxy-b-D-glucopyranoside hydrochloride (HSM1) and its bactericidal effect for a large number of Gram-positive cocci, as well as to investigate its in vitro interaction with seven clinically used antibiotics. In vivo, a wound model was established through the panniculus carnosus of BALB/c mice and then inoculated with 5_107 c.f.u. Staphylococcus aureus or Enterococcus faecalis. For each bacterial strain, the study included an infected or non-infected group that did not receive any treatment, a group treated with local HSM1, a group treated with intraperitoneal vancomycin, a group treated with intraperitoneal daptomycin and two groups that received HSM1 local treatment plus intraperitoneal vancomycin or daptomycin. All isolates were inhibited by HSM1 at concentrations of 2-32 mg l-1. Synergy was demonstrated when HSM1 was combined with vancomycin and daptomycin. In in vivo studies, all groups treated with single drugs showed a statistically significant result compared with the control group. The two groups treated with drug combinations showed the highest antimicrobial efficacy. The good in vitro activities and the in vivo efficacy suggest HSM1 as a promising therapeutic candidate in Gram-positive wound infections.

Original languageEnglish
Pages (from-to)1337-1343
Number of pages7
JournalJournal of Medical Microbiology
Volume60
Issue number9
DOIs
Publication statusPublished - Sep 2011

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

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