In Vitro Activity of Antifungal Drugs Against Trichophyton rubrum and Trichophyton mentagrophytes spp. by E-Test Method and Non-supplemented Mueller–Hinton Agar Plates

Jari Intra, Cecilia Sarto, Selene Mazzola, Chiara Fania, Natalia Tiberti, Paolo Brambilla

Research output: Contribution to journalArticle

Abstract

Trichophyton rubrum and Trichophyton mentagrophytes spp. are two of the most frequently isolated dermatophytes causing dermatophytosis worldwide. Since the incidence of resistance to antifungal agents is increasing, antifungal susceptibility tests are needed to successfully treat dermatophytoses. Most of the methods currently available are complicated, time-consuming and lack of reference procedures. The aim of this work was to establish a simple protocol to test the susceptibility of dermatophytes isolated from clinical samples against five antifungal drugs using E-test and disk diffusion methods. We used the E-test on non-supplemented Mueller–Hinton agar plates to determine the minimum inhibitory concentrations (MICs) of fluconazole, itraconazole, voriconazole and amphotericin B, and disk diffusion method to determine the interpretive MIC of terbinafine. Fifty dermatophytes—10 T. rubrum and 40 T. mentagrophytes spp.—were assessed after only 96 h of colony growth. Terbinafine was the most active antifungal agent with an inhibition diameter greater than 70 mm (sensitivity > 20 mm), followed by voriconazole, itraconazole and amphotericin B with MICs ranging from 0.032 to 0.38 µg/mL, from 0.006 to 0.125 µg/mL and from 0.5 to 1.5 µg/mL, respectively. All isolates were resistant to fluconazole. Collectively, the less laborious E-test and disk diffusion method were shown to be suitable and reliable to determine antifungal sensitivity of dermatophytes. This simple standard protocol could be employed in the routine of clinical laboratories.

Original languageEnglish
Pages (from-to)517-523
Number of pages7
JournalMycopathologia
Volume184
Issue number4
DOIs
Publication statusPublished - Aug 15 2019

Fingerprint

Trichophyton rubrum
Trichophyton mentagrophytes
Trichophyton
antifungal agents
terbinafine
Agar
agar
Arthrodermataceae
keratinophilic fungi
Microbial Sensitivity Tests
minimum inhibitory concentration
dermatomycoses
Tinea
fluconazole
Itraconazole
itraconazole
Pharmaceutical Preparations
Fluconazole
amphotericin B
Antifungal Agents

Keywords

  • Antifungal resistance
  • Antifungal susceptibility
  • Dermatophytosis
  • Fluconazole
  • Terbinafine

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology
  • Agronomy and Crop Science
  • veterinary (miscalleneous)

Cite this

In Vitro Activity of Antifungal Drugs Against Trichophyton rubrum and Trichophyton mentagrophytes spp. by E-Test Method and Non-supplemented Mueller–Hinton Agar Plates. / Intra, Jari; Sarto, Cecilia; Mazzola, Selene; Fania, Chiara; Tiberti, Natalia; Brambilla, Paolo.

In: Mycopathologia, Vol. 184, No. 4, 15.08.2019, p. 517-523.

Research output: Contribution to journalArticle

Intra, Jari ; Sarto, Cecilia ; Mazzola, Selene ; Fania, Chiara ; Tiberti, Natalia ; Brambilla, Paolo. / In Vitro Activity of Antifungal Drugs Against Trichophyton rubrum and Trichophyton mentagrophytes spp. by E-Test Method and Non-supplemented Mueller–Hinton Agar Plates. In: Mycopathologia. 2019 ; Vol. 184, No. 4. pp. 517-523.
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