In vitro activity of prulifloxacin against Escherichia coli isolated from urinary tract infections and the biological cost of prulifloxacin resistance

L. Gualco, A. M. Schito, G. C. Schito, A. Marchese

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Minimum inhibitory concentrations (MICs) and mutant prevention concentrations (MPCs) of prulifloxacin against 30 strains of Escherichia coli isolated from urinary tract infections as well as the 'biological cost' related to acquisition of resistance to the same drug in 10 uropathogenic E. coli were assessed. In terms of MIC90, prulifloxacin was more potent than ciprofloxacin and levofloxacin. Prulifloxacin produced lower or equal MPC values than the other two fluoroquinolones (93.3% and 73.3% compared with levofloxacin and ciprofloxacin, respectively). Compared with susceptible strains, prulifloxacin-resistant mutants showed a reduced rate of growth (ranging from 20.0% to 98.0% in different culture media and incubation conditions) and a decreased fitness index (ranging from 0.959 to 0.999). They were also impaired in their ability to adhere to uroepithelial cells and urinary catheters (11.7-66.4% and 16.3-78.3% reduction, respectively) and showed a lower surface hydrophobicity (51.2-76.0%). They were more susceptible to ultraviolet irradiation (30.6-93.8% excess mortality), showed increased resistance to colicins and diminished transfer of plasmids (-8 vs. 3.3 × 10-7-2.4 × 10-4). Synthesis of haemolysin and type I fimbriae and production of flagella were also adversely affected. This study demonstrates a strict relationship between acquisition of prulifloxacin resistance and loss of important virulence traits. In this transition, E. coli pays a severe biological cost that entails a general reduction of fitness, thus compromising competition with susceptible wild-type strains in the absence of the drug.

Original languageEnglish
Pages (from-to)679-687
Number of pages9
JournalInternational Journal of Antimicrobial Agents
Volume29
Issue number6
DOIs
Publication statusPublished - Jun 2007

Fingerprint

Urinary Tract Infections
Escherichia coli
Costs and Cost Analysis
Levofloxacin
Ciprofloxacin
Uropathogenic Escherichia coli
Colicins
Urinary Catheters
Hemolysin Proteins
Flagella
Fluoroquinolones
Microbial Sensitivity Tests
Hydrophobic and Hydrophilic Interactions
Pharmaceutical Preparations
Virulence
Culture Media
Plasmids
In Vitro Techniques
prulifloxacin
Mortality

Keywords

  • Antimicrobial resistance
  • Escherichia coli
  • Fitness
  • Fluoroquinolones
  • UTI

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Microbiology
  • Parasitology
  • Virology
  • Immunology and Allergy
  • Infectious Diseases

Cite this

In vitro activity of prulifloxacin against Escherichia coli isolated from urinary tract infections and the biological cost of prulifloxacin resistance. / Gualco, L.; Schito, A. M.; Schito, G. C.; Marchese, A.

In: International Journal of Antimicrobial Agents, Vol. 29, No. 6, 06.2007, p. 679-687.

Research output: Contribution to journalArticle

@article{9ec79912f3c249aa9e49a6f50f39ca6f,
title = "In vitro activity of prulifloxacin against Escherichia coli isolated from urinary tract infections and the biological cost of prulifloxacin resistance",
abstract = "Minimum inhibitory concentrations (MICs) and mutant prevention concentrations (MPCs) of prulifloxacin against 30 strains of Escherichia coli isolated from urinary tract infections as well as the 'biological cost' related to acquisition of resistance to the same drug in 10 uropathogenic E. coli were assessed. In terms of MIC90, prulifloxacin was more potent than ciprofloxacin and levofloxacin. Prulifloxacin produced lower or equal MPC values than the other two fluoroquinolones (93.3{\%} and 73.3{\%} compared with levofloxacin and ciprofloxacin, respectively). Compared with susceptible strains, prulifloxacin-resistant mutants showed a reduced rate of growth (ranging from 20.0{\%} to 98.0{\%} in different culture media and incubation conditions) and a decreased fitness index (ranging from 0.959 to 0.999). They were also impaired in their ability to adhere to uroepithelial cells and urinary catheters (11.7-66.4{\%} and 16.3-78.3{\%} reduction, respectively) and showed a lower surface hydrophobicity (51.2-76.0{\%}). They were more susceptible to ultraviolet irradiation (30.6-93.8{\%} excess mortality), showed increased resistance to colicins and diminished transfer of plasmids (-8 vs. 3.3 × 10-7-2.4 × 10-4). Synthesis of haemolysin and type I fimbriae and production of flagella were also adversely affected. This study demonstrates a strict relationship between acquisition of prulifloxacin resistance and loss of important virulence traits. In this transition, E. coli pays a severe biological cost that entails a general reduction of fitness, thus compromising competition with susceptible wild-type strains in the absence of the drug.",
keywords = "Antimicrobial resistance, Escherichia coli, Fitness, Fluoroquinolones, UTI",
author = "L. Gualco and Schito, {A. M.} and Schito, {G. C.} and A. Marchese",
year = "2007",
month = "6",
doi = "10.1016/j.ijantimicag.2007.01.009",
language = "English",
volume = "29",
pages = "679--687",
journal = "International Journal of Antimicrobial Agents",
issn = "0924-8579",
publisher = "Elsevier",
number = "6",

}

TY - JOUR

T1 - In vitro activity of prulifloxacin against Escherichia coli isolated from urinary tract infections and the biological cost of prulifloxacin resistance

AU - Gualco, L.

AU - Schito, A. M.

AU - Schito, G. C.

AU - Marchese, A.

PY - 2007/6

Y1 - 2007/6

N2 - Minimum inhibitory concentrations (MICs) and mutant prevention concentrations (MPCs) of prulifloxacin against 30 strains of Escherichia coli isolated from urinary tract infections as well as the 'biological cost' related to acquisition of resistance to the same drug in 10 uropathogenic E. coli were assessed. In terms of MIC90, prulifloxacin was more potent than ciprofloxacin and levofloxacin. Prulifloxacin produced lower or equal MPC values than the other two fluoroquinolones (93.3% and 73.3% compared with levofloxacin and ciprofloxacin, respectively). Compared with susceptible strains, prulifloxacin-resistant mutants showed a reduced rate of growth (ranging from 20.0% to 98.0% in different culture media and incubation conditions) and a decreased fitness index (ranging from 0.959 to 0.999). They were also impaired in their ability to adhere to uroepithelial cells and urinary catheters (11.7-66.4% and 16.3-78.3% reduction, respectively) and showed a lower surface hydrophobicity (51.2-76.0%). They were more susceptible to ultraviolet irradiation (30.6-93.8% excess mortality), showed increased resistance to colicins and diminished transfer of plasmids (-8 vs. 3.3 × 10-7-2.4 × 10-4). Synthesis of haemolysin and type I fimbriae and production of flagella were also adversely affected. This study demonstrates a strict relationship between acquisition of prulifloxacin resistance and loss of important virulence traits. In this transition, E. coli pays a severe biological cost that entails a general reduction of fitness, thus compromising competition with susceptible wild-type strains in the absence of the drug.

AB - Minimum inhibitory concentrations (MICs) and mutant prevention concentrations (MPCs) of prulifloxacin against 30 strains of Escherichia coli isolated from urinary tract infections as well as the 'biological cost' related to acquisition of resistance to the same drug in 10 uropathogenic E. coli were assessed. In terms of MIC90, prulifloxacin was more potent than ciprofloxacin and levofloxacin. Prulifloxacin produced lower or equal MPC values than the other two fluoroquinolones (93.3% and 73.3% compared with levofloxacin and ciprofloxacin, respectively). Compared with susceptible strains, prulifloxacin-resistant mutants showed a reduced rate of growth (ranging from 20.0% to 98.0% in different culture media and incubation conditions) and a decreased fitness index (ranging from 0.959 to 0.999). They were also impaired in their ability to adhere to uroepithelial cells and urinary catheters (11.7-66.4% and 16.3-78.3% reduction, respectively) and showed a lower surface hydrophobicity (51.2-76.0%). They were more susceptible to ultraviolet irradiation (30.6-93.8% excess mortality), showed increased resistance to colicins and diminished transfer of plasmids (-8 vs. 3.3 × 10-7-2.4 × 10-4). Synthesis of haemolysin and type I fimbriae and production of flagella were also adversely affected. This study demonstrates a strict relationship between acquisition of prulifloxacin resistance and loss of important virulence traits. In this transition, E. coli pays a severe biological cost that entails a general reduction of fitness, thus compromising competition with susceptible wild-type strains in the absence of the drug.

KW - Antimicrobial resistance

KW - Escherichia coli

KW - Fitness

KW - Fluoroquinolones

KW - UTI

UR - http://www.scopus.com/inward/record.url?scp=34248197543&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34248197543&partnerID=8YFLogxK

U2 - 10.1016/j.ijantimicag.2007.01.009

DO - 10.1016/j.ijantimicag.2007.01.009

M3 - Article

C2 - 17363225

AN - SCOPUS:34248197543

VL - 29

SP - 679

EP - 687

JO - International Journal of Antimicrobial Agents

JF - International Journal of Antimicrobial Agents

SN - 0924-8579

IS - 6

ER -