In vitro analysis of early genotoxic and cytotoxic effects of okadaic acid in different cell types of the mussel mytilus galloprovincialis

María Verónica Prego-Faraldo, Vanessa Valdiglesias, Blanca Laffon, José M. Eirín-López, Josefina Méndez

Research output: Contribution to journalArticle

Abstract

Okadaic acid (OA) is the predominant biotoxin responsible for diarrhetic shellfish poisoning (DSP) syndrome in humans. While its harmful effects have been extensively studied in mammalian cell lines, the impact on marine organisms routinely exposed to OA is still not fully known. Few investigations available on bivalve molluscs suggest less genotoxic and cytotoxic effects of OA at high concentrations during long exposure times. In contrast, no apparent information is available on how sublethal concentrations of OA affect these organisms over short exposure times. In order to fill this gap, this study addressed for the first time in vitro analysis of early genotoxic and cytotoxic effects attributed to OA in two cell types of the mussel Mytilus galloprovincialis. Accordingly, hemocytes and gill cells were exposed to low OA concentrations (10, 50, 100, 200, or 500 nM) for short periods of time (1 or 2 h). The resulting DNA damage, as apoptosis and necrosis, was subsequently quantified using the comet assay and flow cytometry, respectively. Data demonstrated that (1) mussel hemocytes seem to display a resistance mechanism against early genotoxic and cytotoxic OA-induced effects, (2) mussel gill cells display higher sensitivity to early OA-mediated genotoxicity than hemocytes, and (3) mussel gill cells constitute more suitable systems to evaluate the genotoxic effect of low OA concentrations in short exposure studies. Taken together, this investigation provides evidence supporting the more reliable suitability of mussel gill cells compared to hemocytes to evaluate the genotoxic effect of low short-duration exposure to OA.

Original languageEnglish
Pages (from-to)814-824
Number of pages11
JournalJournal of Toxicology and Environmental Health - Part A: Current Issues
Volume78
Issue number13
DOIs
Publication statusPublished - Jan 1 2015

ASJC Scopus subject areas

  • Health, Toxicology and Mutagenesis
  • Toxicology

Fingerprint Dive into the research topics of 'In vitro analysis of early genotoxic and cytotoxic effects of okadaic acid in different cell types of the mussel mytilus galloprovincialis'. Together they form a unique fingerprint.

  • Cite this