In vitro analysis of low-level laser irradiation on human osteoblast-like cells proliferation

Nora Bloise, Enrica Saino, Francesca Bragheri, Paolo Minzioni, Ilaria Cristiani, Marcello Imbriani, Livia Visai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The objective of this study was to examine the in vitro effect of a single or a multiple doses of low-level laser irradiation (LLLI) on proliferation of the human osteosarcoma cell line, SAOS-2. SAOS-2 cells were divided in five groups and exposed to LLLI (659 nm diode laser; 11 mW power output): group I as a control (dark), group II exposed to a single laser dose of 1 J/cm2, group III irradiated with a single dose of 3 J/cm2, and group IV and V exposed for three consecutive days to 1 or 3 J/cm2, respectively. Cellular proliferation was assessed daily up to 7 days of culturing. The obtained results showed an increase in proliferative capacity of SAOS-2 cells during the first 96 h of culturing time in once-irradiated cells, as compared to control cells. Furthermore, a significantly higher proliferation in the group IV and V was detected if compared to a single dose or to control group after 96 h and 7 days. In conclusion, the effect of the single dose on cell proliferation was transitory and repeated irradiations were necessary to observe a strong enhancement of SAOS-2 growth. As a future perspective, we would like to determine the potential of LLLI as a new approach for promoting bone regeneration onto biomaterials.

Original languageEnglish
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8092
DOIs
Publication statusPublished - 2011
EventMedical Laser Applications and Laser-Tissue Interactions V - Munich, Germany
Duration: May 24 2011May 26 2011

Other

OtherMedical Laser Applications and Laser-Tissue Interactions V
CountryGermany
CityMunich
Period5/24/115/26/11

Fingerprint

osteoblasts
Osteoblasts
Cell proliferation
Laser beam effects
Lasers
Cell Proliferation
Dosimetry
irradiation
Cells
dosage
lasers
Biocompatible Materials
Biomaterials
Semiconductor Lasers
Control Groups
Bone Regeneration
Semiconductor lasers
Bone
Osteosarcoma
Irradiation

Keywords

  • Cell Proliferation
  • Low-Level Laser Irradiation (LLLI)
  • SAOS-2 human osteoblast-like cells

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Bloise, N., Saino, E., Bragheri, F., Minzioni, P., Cristiani, I., Imbriani, M., & Visai, L. (2011). In vitro analysis of low-level laser irradiation on human osteoblast-like cells proliferation. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 8092). [80920U] https://doi.org/10.1117/12.889873

In vitro analysis of low-level laser irradiation on human osteoblast-like cells proliferation. / Bloise, Nora; Saino, Enrica; Bragheri, Francesca; Minzioni, Paolo; Cristiani, Ilaria; Imbriani, Marcello; Visai, Livia.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 8092 2011. 80920U.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Bloise, N, Saino, E, Bragheri, F, Minzioni, P, Cristiani, I, Imbriani, M & Visai, L 2011, In vitro analysis of low-level laser irradiation on human osteoblast-like cells proliferation. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 8092, 80920U, Medical Laser Applications and Laser-Tissue Interactions V, Munich, Germany, 5/24/11. https://doi.org/10.1117/12.889873
Bloise N, Saino E, Bragheri F, Minzioni P, Cristiani I, Imbriani M et al. In vitro analysis of low-level laser irradiation on human osteoblast-like cells proliferation. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 8092. 2011. 80920U https://doi.org/10.1117/12.889873
Bloise, Nora ; Saino, Enrica ; Bragheri, Francesca ; Minzioni, Paolo ; Cristiani, Ilaria ; Imbriani, Marcello ; Visai, Livia. / In vitro analysis of low-level laser irradiation on human osteoblast-like cells proliferation. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 8092 2011.
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