In Vitro analysis of low-level laser irradiation on human osteoblastlike cells proliferation

Nora Bloise, Enrica Saino, Francesca Bragheri, Paolo Minzioni, Ilaria Cristiani, Marcello Imbriani, Livia Visai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The objective of this study was to examine the in vitro effect of a single or a multiple doses of low-level laser irradiation (LLLI) on proliferation of the human osteosarcoma cell line, SAOS-2. SAOS-2 cells were divided in five groups and exposed to LLLI (659 nm diode laser; 11 mW power output): group I as a control (dark), group II exposed to a single laser dose of 1 J/cm2, group III irradiated with a single dose of 3 J/cm2, and group IV and V exposed for three consecutive days to 1 or 3 J/cm2, respectively. Cellular proliferation was assessed daily up to 7 days of culturing. The obtained results showed an increase in proliferative capacity of SAOS-2 cells during the first 96 h of culturing time in once-irradiated cells, as compared to control cells. Furthermore, a significantly higher proliferation in the group IV and V was detected if compared to a single dose or to control group after 96 h and 7 days. In conclusion, the effect of the single dose on cell proliferation was transitory and repeated irradiations were necessary to observe a strong enhancement of SAOS-2 growth. As a future perspective, we would like to determine the potential of LLLI as a new approach for promoting bone regeneration onto biomaterials.

Original languageEnglish
Title of host publicationOptics InfoBase Conference Papers
Publication statusPublished - 2011
EventEuropean Conference on Biomedical Optics, ECBO 2011 - Munich, Germany
Duration: May 22 2011May 26 2011

Other

OtherEuropean Conference on Biomedical Optics, ECBO 2011
CountryGermany
CityMunich
Period5/22/115/26/11

Fingerprint

Cell proliferation
Laser beam effects
Dosimetry
irradiation
Cells
dosage
lasers
Biomaterials
Semiconductor lasers
Bone
Irradiation
cells
Lasers
regeneration
cultured cells
bones
semiconductor lasers
augmentation
output

Keywords

  • Cell proliferation
  • Low-Level laser irradiation (LLLI)
  • SAOS-2 human osteoblast-like cells

ASJC Scopus subject areas

  • Instrumentation
  • Atomic and Molecular Physics, and Optics

Cite this

Bloise, N., Saino, E., Bragheri, F., Minzioni, P., Cristiani, I., Imbriani, M., & Visai, L. (2011). In Vitro analysis of low-level laser irradiation on human osteoblastlike cells proliferation. In Optics InfoBase Conference Papers [80920U]

In Vitro analysis of low-level laser irradiation on human osteoblastlike cells proliferation. / Bloise, Nora; Saino, Enrica; Bragheri, Francesca; Minzioni, Paolo; Cristiani, Ilaria; Imbriani, Marcello; Visai, Livia.

Optics InfoBase Conference Papers. 2011. 80920U.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Bloise, N, Saino, E, Bragheri, F, Minzioni, P, Cristiani, I, Imbriani, M & Visai, L 2011, In Vitro analysis of low-level laser irradiation on human osteoblastlike cells proliferation. in Optics InfoBase Conference Papers., 80920U, European Conference on Biomedical Optics, ECBO 2011, Munich, Germany, 5/22/11.
Bloise N, Saino E, Bragheri F, Minzioni P, Cristiani I, Imbriani M et al. In Vitro analysis of low-level laser irradiation on human osteoblastlike cells proliferation. In Optics InfoBase Conference Papers. 2011. 80920U
Bloise, Nora ; Saino, Enrica ; Bragheri, Francesca ; Minzioni, Paolo ; Cristiani, Ilaria ; Imbriani, Marcello ; Visai, Livia. / In Vitro analysis of low-level laser irradiation on human osteoblastlike cells proliferation. Optics InfoBase Conference Papers. 2011.
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AU - Cristiani, Ilaria

AU - Imbriani, Marcello

AU - Visai, Livia

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