In vitro and in vivo angiogenic activity of osteoarthritic and osteoporotic osteoblasts is modulated by VEGF and vitamin D3 treatment

Anna Neve, Francesco Paolo Cantatore, Addolorata Corrado, Annamaria Gaudio, Simona Ruggieri, Domenico Ribatti

Research output: Contribution to journalArticlepeer-review

Abstract

Vascular Endothelial Growth Factor (VEGF) is a potent angiogenic factor, which also regulates bone remodeling. Osteoblasts not only respond to VEGF stimulation, but also express and synthesize this factor. The present study was aimed to evaluate in vitro differences in VEGF production and expression of cultured human osteoblastic cells derived from healthy donors and from subjects affected by osteoarthritis and osteoporosis, under basal conditions than after vitamin D3, and to investigate the angiogenic activity of culture media obtained by these cells in chick embryo chorioallantoic membrane (CAM) assay. The results showed that normal and pathological osteoblasts produce and express VEGF and 1,25 dihydroxy-vitamin D3 treatment increases protein and m-RNA VEGF levels. In addition culture media of pathological osteoblasts induce a strong angiogenic response, greater than observed with culture medium of normal cells, suggesting the involvement of osteoblast-derived VEGF in the pathogenesis of bone diseases. Human osteoblastic cells from subjects affected by osteoarthritis and osteoporosis produce VEGF.Vitamin D enhances VEGF production in normal and pathological osteoblasts.Osteoblasts-derived VEGF promotes angiogenesis in vivo.

Original languageEnglish
Pages (from-to)81-84
Number of pages4
JournalRegulatory Peptides
Volume184
DOIs
Publication statusPublished - Jun 2013

Keywords

  • Angiogenesis
  • Chorioallantoic membrane
  • Osteoarthritis
  • Osteoporosis
  • Vitamin D3

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

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