In vitro and in vivo cytotoxicity of 6 amino-5-formylmethylamino-1,3-dimethyl uracil, a uracilic metabolite of caffeine

S. Garattini, E. Erba, L. Morasca, G. Peri, A. Mantovani, S. Filippeschi, F. Spreafico, M. Arnaud

Research output: Contribution to journalArticlepeer-review

Abstract

The in vitro cytotoxicity of 6 amino-5 formylmethylamino-1,3 dimethyluracil (ADMU) a major metabolite of caffeine in rats was studied by cell counts or [3H]thymidine incorporation in the murine Lewis lung carcinoma (3LL) and L929 fibroblast cells and in the human E cell line derived from an ovarian carcinoma. Unlike 5-fluorouracil (5FU) which was markedly cytotoxic, ADMU concentrations up to 60 μg/ml were devoid appreciable cytocidal action. Similarly, 1-10 μg 5FU markedly inhibited the blastogenic response of rat lymphocytes to PHA, whereas lymphoproliferation was not affected at ADMU concentrations up to 100 μg/ml. In vivo administration of ADMU (40 mg/kg, twice a day on day 1 to 3) to L1210 leukaemia-bearing mice caused a transient short-lasting reduction of tumour cell numbers only on day 6 after leukaemia inoculation.

Original languageEnglish
Pages (from-to)313-319
Number of pages7
JournalToxicology Letters
Volume10
Issue number2-3
DOIs
Publication statusPublished - 1982

ASJC Scopus subject areas

  • Toxicology

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