In-vitro and in-vivo detection of p53 by fluorescence lifetime on a hybrid FITC-gold nanosensor

L. Sironi; S. Freddi; L. D'Alfonso; M. Collini; T. Gorletta; S. Soddu; G. Chirico

doi:10.1117/12.841094

In-vitro and in-vivo detection of p53 by fluorescence lifetime on a hybrid FITC-gold nanosensor

L. Sironi, S. Freddi, L. D'Alfonso, M. Collini, T. Gorletta, S. Soddu, G. Chirico

Istituto Regina Elena

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

P53 is a tumor suppressor used as marker for early cancer diagnosis and prognosis. We have studied constructs based on gold nanoparticles (NPs) decorated with specific anti-p53 antibodies and with a fluoresceine derivative, FITC. The interaction of gold surface plasmons with fluorophores bound within few nanometers from the surface, likely induces changes in the fluorophore excited state lifetime. Indeed we found previously that this parameter follows linearly the p53 concentration in solutions (in vitro conditions) up to 200-400 pM, depending on the size of the NP, with a 5 pM uncertainty. We have evaluated here the nanosensor specificity for p53 by testing it in-vitro against bovine serum albumine, beta-lactolglobulin and lysozyme. Moreover, the titration of total cell extracts from p53+/+ or p53-/- cells with the p53antibody decorated gold NPs, indicates that this construct can also be used to detect the presence of p53 in total cell extracts and it will be therefore a valuable tool also for in vivo screening.

Original language	English
Title of host publication	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	7574
DOIs	https://doi.org/10.1117/12.841094
Publication status	Published - 2010
Event	Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VII - San Francisco, CA, United States Duration: Jan 25 2010 → Jan 28 2010

Other

Other	Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VII
Country	United States
City	San Francisco, CA
Period	1/25/10 → 1/28/10

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics
Electronic, Optical and Magnetic Materials
Biomaterials
Radiology Nuclear Medicine and imaging

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Sironi, L, Freddi, S, D'Alfonso, L, Collini, M, Gorletta, T, Soddu, S & Chirico, G 2010, In-vitro and in-vivo detection of p53 by fluorescence lifetime on a hybrid FITC-gold nanosensor. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 7574, 757403, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VII, San Francisco, CA, United States, 1/25/10. https://doi.org/10.1117/12.841094

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abstract = "P53 is a tumor suppressor used as marker for early cancer diagnosis and prognosis. We have studied constructs based on gold nanoparticles (NPs) decorated with specific anti-p53 antibodies and with a fluoresceine derivative, FITC. The interaction of gold surface plasmons with fluorophores bound within few nanometers from the surface, likely induces changes in the fluorophore excited state lifetime. Indeed we found previously that this parameter follows linearly the p53 concentration in solutions (in vitro conditions) up to 200-400 pM, depending on the size of the NP, with a 5 pM uncertainty. We have evaluated here the nanosensor specificity for p53 by testing it in-vitro against bovine serum albumine, beta-lactolglobulin and lysozyme. Moreover, the titration of total cell extracts from p53+/+ or p53-/- cells with the p53antibody decorated gold NPs, indicates that this construct can also be used to detect the presence of p53 in total cell extracts and it will be therefore a valuable tool also for in vivo screening.",

author = "L. Sironi and S. Freddi and L. D'Alfonso and M. Collini and T. Gorletta and S. Soddu and G. Chirico",

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AU - Sironi, L.

AU - Freddi, S.

AU - D'Alfonso, L.

AU - Collini, M.

AU - Gorletta, T.

AU - Soddu, S.

AU - Chirico, G.

PY - 2010

Y1 - 2010

N2 - P53 is a tumor suppressor used as marker for early cancer diagnosis and prognosis. We have studied constructs based on gold nanoparticles (NPs) decorated with specific anti-p53 antibodies and with a fluoresceine derivative, FITC. The interaction of gold surface plasmons with fluorophores bound within few nanometers from the surface, likely induces changes in the fluorophore excited state lifetime. Indeed we found previously that this parameter follows linearly the p53 concentration in solutions (in vitro conditions) up to 200-400 pM, depending on the size of the NP, with a 5 pM uncertainty. We have evaluated here the nanosensor specificity for p53 by testing it in-vitro against bovine serum albumine, beta-lactolglobulin and lysozyme. Moreover, the titration of total cell extracts from p53+/+ or p53-/- cells with the p53antibody decorated gold NPs, indicates that this construct can also be used to detect the presence of p53 in total cell extracts and it will be therefore a valuable tool also for in vivo screening.

AB - P53 is a tumor suppressor used as marker for early cancer diagnosis and prognosis. We have studied constructs based on gold nanoparticles (NPs) decorated with specific anti-p53 antibodies and with a fluoresceine derivative, FITC. The interaction of gold surface plasmons with fluorophores bound within few nanometers from the surface, likely induces changes in the fluorophore excited state lifetime. Indeed we found previously that this parameter follows linearly the p53 concentration in solutions (in vitro conditions) up to 200-400 pM, depending on the size of the NP, with a 5 pM uncertainty. We have evaluated here the nanosensor specificity for p53 by testing it in-vitro against bovine serum albumine, beta-lactolglobulin and lysozyme. Moreover, the titration of total cell extracts from p53+/+ or p53-/- cells with the p53antibody decorated gold NPs, indicates that this construct can also be used to detect the presence of p53 in total cell extracts and it will be therefore a valuable tool also for in vivo screening.

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