In-vitro and in-vivo detection of p53 by fluorescence lifetime on a hybrid FITC-gold nanosensor

L. Sironi, S. Freddi, L. D'Alfonso, M. Collini, T. Gorletta, S. Soddu, G. Chirico

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

P53 is a tumor suppressor used as marker for early cancer diagnosis and prognosis. We have studied constructs based on gold nanoparticles (NPs) decorated with specific anti-p53 antibodies and with a fluoresceine derivative, FITC. The interaction of gold surface plasmons with fluorophores bound within few nanometers from the surface, likely induces changes in the fluorophore excited state lifetime. Indeed we found previously that this parameter follows linearly the p53 concentration in solutions (in vitro conditions) up to 200-400 pM, depending on the size of the NP, with a 5 pM uncertainty. We have evaluated here the nanosensor specificity for p53 by testing it in-vitro against bovine serum albumine, beta-lactolglobulin and lysozyme. Moreover, the titration of total cell extracts from p53+/+ or p53-/- cells with the p53antibody decorated gold NPs, indicates that this construct can also be used to detect the presence of p53 in total cell extracts and it will be therefore a valuable tool also for in vivo screening.

Original languageEnglish
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7574
DOIs
Publication statusPublished - 2010
EventNanoscale Imaging, Sensing, and Actuation for Biomedical Applications VII - San Francisco, CA, United States
Duration: Jan 25 2010Jan 28 2010

Other

OtherNanoscale Imaging, Sensing, and Actuation for Biomedical Applications VII
CountryUnited States
CitySan Francisco, CA
Period1/25/101/28/10

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Fingerprint Dive into the research topics of 'In-vitro and in-vivo detection of p53 by fluorescence lifetime on a hybrid FITC-gold nanosensor'. Together they form a unique fingerprint.

Cite this