Abstract
Objective: The effects of metformin on adiponectin production are controversial and have never been investigated in human adipose tissue. We analysed whether metformin modulates, in vitro and in vivo, gene expression, protein content, and secretion of adiponectin. Methods: For the in vitro study, subcutaneous adipose tissue (SAT) and visceral adipose tissue (VAT) samples from 5 non-diabetic obese patients were collected. For the in vivo investigation, 22 obese patients were randomly assigned to metformin+lifestyle (ML) or placebo+lifestyle (PL) intervention. SAT specimens and blood samples were collected before and after the intervention in both groups. Results: In in vitro experiments, treatment with metformin increased the expression and secretion of adiponectin in SAT, but not in VAT explants. In the in vivo study, a significant increase in adiponectin and a decreased expression of a macrophage activation marker (CD68) were observed only in SAT of the ML group. Conclusion: These results demonstrate that metformin is able to up-regulate adiponectin gene expression, both in vivo and in vitro, and to stimulate adiponectin protein secretion from human SAT in vitro. It could be hypothesised that metformin-induced adiponectin increase within adipose tissue may have an unexpected role in the reduction of local inflammation.
| Original language | English |
|---|---|
| Pages (from-to) | 27-33 |
| Number of pages | 7 |
| Journal | Obesity Facts |
| Volume | 4 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Feb 2011 |
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Keywords
- Adiponectin
- Adipose tissue
- Insulin resistance
- Metformin
- Obesity
ASJC Scopus subject areas
- Physiology (medical)
- Health(social science)
Cite this
In vitro and in vivo effects of metformin on human adipose tissue adiponectin. / Zulian, Alessandra; Cancello, Raffaella; Girola, Andrea; Gilardini, Luisa; Alberti, Luisella; Croci, Marina; Micheletto, Giancarlo; Danelli, Piergiorgio; Invitti, Cecilia.
In: Obesity Facts, Vol. 4, No. 1, 02.2011, p. 27-33.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - In vitro and in vivo effects of metformin on human adipose tissue adiponectin
AU - Zulian, Alessandra
AU - Cancello, Raffaella
AU - Girola, Andrea
AU - Gilardini, Luisa
AU - Alberti, Luisella
AU - Croci, Marina
AU - Micheletto, Giancarlo
AU - Danelli, Piergiorgio
AU - Invitti, Cecilia
PY - 2011/2
Y1 - 2011/2
N2 - Objective: The effects of metformin on adiponectin production are controversial and have never been investigated in human adipose tissue. We analysed whether metformin modulates, in vitro and in vivo, gene expression, protein content, and secretion of adiponectin. Methods: For the in vitro study, subcutaneous adipose tissue (SAT) and visceral adipose tissue (VAT) samples from 5 non-diabetic obese patients were collected. For the in vivo investigation, 22 obese patients were randomly assigned to metformin+lifestyle (ML) or placebo+lifestyle (PL) intervention. SAT specimens and blood samples were collected before and after the intervention in both groups. Results: In in vitro experiments, treatment with metformin increased the expression and secretion of adiponectin in SAT, but not in VAT explants. In the in vivo study, a significant increase in adiponectin and a decreased expression of a macrophage activation marker (CD68) were observed only in SAT of the ML group. Conclusion: These results demonstrate that metformin is able to up-regulate adiponectin gene expression, both in vivo and in vitro, and to stimulate adiponectin protein secretion from human SAT in vitro. It could be hypothesised that metformin-induced adiponectin increase within adipose tissue may have an unexpected role in the reduction of local inflammation.
AB - Objective: The effects of metformin on adiponectin production are controversial and have never been investigated in human adipose tissue. We analysed whether metformin modulates, in vitro and in vivo, gene expression, protein content, and secretion of adiponectin. Methods: For the in vitro study, subcutaneous adipose tissue (SAT) and visceral adipose tissue (VAT) samples from 5 non-diabetic obese patients were collected. For the in vivo investigation, 22 obese patients were randomly assigned to metformin+lifestyle (ML) or placebo+lifestyle (PL) intervention. SAT specimens and blood samples were collected before and after the intervention in both groups. Results: In in vitro experiments, treatment with metformin increased the expression and secretion of adiponectin in SAT, but not in VAT explants. In the in vivo study, a significant increase in adiponectin and a decreased expression of a macrophage activation marker (CD68) were observed only in SAT of the ML group. Conclusion: These results demonstrate that metformin is able to up-regulate adiponectin gene expression, both in vivo and in vitro, and to stimulate adiponectin protein secretion from human SAT in vitro. It could be hypothesised that metformin-induced adiponectin increase within adipose tissue may have an unexpected role in the reduction of local inflammation.
KW - Adiponectin
KW - Adipose tissue
KW - Insulin resistance
KW - Metformin
KW - Obesity
UR - http://www.scopus.com/inward/record.url?scp=79952384985&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79952384985&partnerID=8YFLogxK
U2 - 10.1159/000324582
DO - 10.1159/000324582
M3 - Article
C2 - 21372608
AN - SCOPUS:79952384985
VL - 4
SP - 27
EP - 33
JO - Obesity Facts
JF - Obesity Facts
SN - 1662-4025
IS - 1
ER -