In vitro and in vivo enzyme-mediated biomineralization of oligo(poly(ethylene glycol) fumarate hydrogels

Matilde Bongio, M. Reza Nejadnik, Z. Tahmasebi Birgani, Pamela Habibovic, Lucas A. Kinard, F. Kurtis Kasper, Antonios G. Mikos, John A. Jansen, Sander C G Leeuwenburgh, Jeroen J J P Van Den Beucken

Research output: Contribution to journalArticlepeer-review

Abstract

The enzyme alkaline phosphatase (ALP) is added at different concentrations (i.e., 0, 2.5, and 10mg·ml-1) to oligo(poly(ethylene glycol)fumarate) (OPF) hydrogels. The scaffolds are either incubated in 10mM calcium glycerophosphate (Ca-GP) solution for 2 weeks or implanted in a rat subcutaneous model for 4 weeks. Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), and alizarin red staining show a strong ability to form minerals exclusively in ALP-containing hydrogels in vitro. Additionally, the calcium content increases with increasing ALP concentration. Similarly, only ALP-containing hydrogels induce mineralization in vivo. Specifically, small (≈5-20μm) mineral deposits are observed at the periphery of the hydrogels near the dermis/scaffold interface using Von Kossa and alizarin red staining.

Original languageEnglish
Pages (from-to)777-788
Number of pages12
JournalMacromolecular Bioscience
Volume13
Issue number6
DOIs
Publication statusPublished - Jun 2013

Keywords

  • Alkaline phosphatase
  • Biomineralization
  • Bone substitutes
  • Hydrogels
  • In vivo

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

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