In vitro and in vivo evidence for the existence of presynaptic muscarinic cholinergic receptors in the rat hippocampus

Silvana Consolo, Jian Xin Wang, Rossella Fusi, Raffaella Vinci, Gianluigi Forloni, Herbert Ladinsky

Research output: Contribution to journalArticle

Abstract

The intrahippocampal injection of kainic acid cleared 50% of muscarinic receptors and favored the detection of a further 20% loss in hippocampal presynaptic muscarinic receptors produced by electrolytic lesion of the medial septal nucleus as determined by Scatchard analysis of the saturation isotherms of [3H]dexetimide binding. In accordance, a decrease of about 20% in the in vivo accumulation of [3H]dexetimide in the hippocampus was found in animals lesioned in the medial septal nucleus. This effect occurred at both the dose of 5 μg/kg and at the saturating dose of 100 μg/kg of [3H]dexetimide. The results suggest that the loss was due to decreased receptor number rather than decreased receptor affinity.

Original languageEnglish
Pages (from-to)147-151
Number of pages5
JournalBrain Research
Volume309
Issue number1
DOIs
Publication statusPublished - Aug 20 1984

Keywords

  • hippocampus
  • kainic acid
  • medial septal lesion
  • presynaptic muscarinic receptor
  • [H]dexetimide

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Fingerprint Dive into the research topics of 'In vitro and in vivo evidence for the existence of presynaptic muscarinic cholinergic receptors in the rat hippocampus'. Together they form a unique fingerprint.

  • Cite this