In vitro and in vivo induction of nitric oxide by murine macrophages stimulated with Bordetella pertussis

Donate Torre, Giulio Ferrario, Giancarla Bonetta, Lorenza Perversi, Filippo Speranza

Research output: Contribution to journalArticlepeer-review

Abstract

Nitric oxide (NO) exhibits potent antimicrobial activity in vitro. The function of NO in host defenses in vivo, however, is presently unclear. Experiments were undertaken to determine the production of NO in vitro from murine peritoneal and alveolar macrophages, and murine macrophage cell line (J774A.1) stimulated with Bordetella pertussis or pertussis toxin (PT). In addition, we determined circulating levels of NO in the sera and bronchoalveolar lavage (BAL) fluids of mice infected intranasally with B. pertussis. The results of this study showed that in vitro murine peritoneal macrophages induce production of NO in response to B. pertussis and PT. In addition, murine macrophage cell line, J774A.1 also induces NO production after stimulation with B. pertussis. NO production was also detected in alveolar macrophages from mice infected intranasally with B. pertussis. Finally, a significant increment of circulating levels of NO was noted, in the sera but not in the BAL fluids, of mice infected intranasally with B. pertussis.

Original languageEnglish
Pages (from-to)95-99
Number of pages5
JournalFEMS Immunology and Medical Microbiology
Volume13
Issue number2
DOIs
Publication statusPublished - Feb 1996

Keywords

  • Bordetella pertussis
  • Murine macrophage
  • Nitric oxide

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Infectious Diseases

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