TY - JOUR
T1 - In vitro and in vivo induction of nitric oxide by murine macrophages stimulated with Bordetella pertussis
AU - Torre, Donate
AU - Ferrario, Giulio
AU - Bonetta, Giancarla
AU - Perversi, Lorenza
AU - Speranza, Filippo
PY - 1996/2
Y1 - 1996/2
N2 - Nitric oxide (NO) exhibits potent antimicrobial activity in vitro. The function of NO in host defenses in vivo, however, is presently unclear. Experiments were undertaken to determine the production of NO in vitro from murine peritoneal and alveolar macrophages, and murine macrophage cell line (J774A.1) stimulated with Bordetella pertussis or pertussis toxin (PT). In addition, we determined circulating levels of NO in the sera and bronchoalveolar lavage (BAL) fluids of mice infected intranasally with B. pertussis. The results of this study showed that in vitro murine peritoneal macrophages induce production of NO in response to B. pertussis and PT. In addition, murine macrophage cell line, J774A.1 also induces NO production after stimulation with B. pertussis. NO production was also detected in alveolar macrophages from mice infected intranasally with B. pertussis. Finally, a significant increment of circulating levels of NO was noted, in the sera but not in the BAL fluids, of mice infected intranasally with B. pertussis.
AB - Nitric oxide (NO) exhibits potent antimicrobial activity in vitro. The function of NO in host defenses in vivo, however, is presently unclear. Experiments were undertaken to determine the production of NO in vitro from murine peritoneal and alveolar macrophages, and murine macrophage cell line (J774A.1) stimulated with Bordetella pertussis or pertussis toxin (PT). In addition, we determined circulating levels of NO in the sera and bronchoalveolar lavage (BAL) fluids of mice infected intranasally with B. pertussis. The results of this study showed that in vitro murine peritoneal macrophages induce production of NO in response to B. pertussis and PT. In addition, murine macrophage cell line, J774A.1 also induces NO production after stimulation with B. pertussis. NO production was also detected in alveolar macrophages from mice infected intranasally with B. pertussis. Finally, a significant increment of circulating levels of NO was noted, in the sera but not in the BAL fluids, of mice infected intranasally with B. pertussis.
KW - Bordetella pertussis
KW - Murine macrophage
KW - Nitric oxide
UR - http://www.scopus.com/inward/record.url?scp=0029878779&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029878779&partnerID=8YFLogxK
U2 - 10.1016/0928-8244(95)00089-5
DO - 10.1016/0928-8244(95)00089-5
M3 - Article
C2 - 8731016
AN - SCOPUS:0029878779
VL - 13
SP - 95
EP - 99
JO - FEMS Immunology and Medical Microbiology
JF - FEMS Immunology and Medical Microbiology
SN - 0928-8244
IS - 2
ER -