In vitro and in vivo inhibition of SK-N-MC neuroblastoma growth using cyclic nucleotide phosphodiesterase inhibitors

M. Giorgi, C. Leonetti, G. Citro, G. Augusti-Tocco

Research output: Contribution to journalArticle

Abstract

The effect of cyclic nucleotide phosphodieterase (PDE) inhibitors Zaprinast and DC-TA-46 has been tested on SK-N-MC neuroblastoma growth. Antiproliferative activity of the tested drugs was assayed both in vitro and in the xenograft model of nude mice. In clonal density experiments, the IC50 value was 3.3 μM for Zaprinast and 1.9 μM for DC-TA-46, while 7.5 μM BCNU alkylating agent was required to obtain the same effect. SK-N-MC cells xenografted in the nude mouse showed that the administration of Zaprinast and DC-TA-46 caused a significant 50% decrease of the tumour weight. These data demonstrate that PDE inhibitors may be useful for at least reducing tumour growth; they may be of interest for further evaluation as alternative molecules in the design of multiple agent protocols for neuroblastoma treatment.

Original languageEnglish
Pages (from-to)25-31
Number of pages7
JournalJournal of Neuro-Oncology
Volume51
Issue number1
DOIs
Publication statusPublished - 2001

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Keywords

  • Cyclic nucleotide phosphodiesterase
  • DC-TA-46
  • Growth inhibition
  • Human neuroblastoma
  • Nude mice
  • Zaprinast

ASJC Scopus subject areas

  • Clinical Neurology
  • Cancer Research
  • Oncology
  • Neuroscience(all)

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